Recombinant Human VIP protein(Met1-Lys169), His-tagged

• Cat.No.: VIP-376H
• Product Overview: Recombinant Human VIP (P01282-2) (Met1-Lys169) with a C-terminal polyhistidine tag was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: His
• Protein length: Met1-Lys169
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The recombinant human VIP comprises 160 amino acids and has a predicted molecular mass of 18.3 kDa. The apparent molecular mass of the protein is approximately 25, 21 and 15 kDa in SDS-PAGE under reducing conditions
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: VIP vasoactive intestinal peptide [ Homo sapiens ]
• Official Symbol: VIP
• Synonyms: VIP; vasoactive intestinal peptide; VIP peptides; PHM27; MGC13587
• Gene ID: 7432
• mRNA Refseq: NM_003381
• Protein Refseq: NP_003372
• MIM: 192320
• UniProt ID: P01282