Recombinant Human VIM Protein, His-tagged

• Cat.No.: VIM-571H
• Product Overview: Recombinant Human VIM Protein (Glu286-Glu408) with N-His tag was expressed in E. coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: Glu286-Glu408
• Description: This gene encodes a type III intermediate filament protein. Intermediate filaments, along with microtubules and actin microfilaments, make up the cytoskeleton. The encoded protein is responsible for maintaining cell shape and integrity of the cytoplasm, and stabilizing cytoskeletal interactions. This protein is involved in neuritogenesis and cholesterol transport and functions as an organizer of a number of other critical proteins involved in cell attachment, migration, and signaling. Bacterial and viral pathogens have been shown to attach to this protein on the host cell surface. Mutations in this gene are associated with congenital cataracts in human patients.
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 22.5 kDa
  Accurate Molecular Mass: 25 kDa
• Purity: > 90%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: PBS, pH7.4, containing 0.01% SKL, 1 mM DTT, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in sterile water to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: VIM vimentin [ Homo sapiens (human) ]
• Official Symbol: VIM
• Synonyms: VIM; vimentin; FLJ36605; epididymis secretory sperm binding protein
• Gene ID: 7431
• mRNA Refseq: NM_003380
• Protein Refseq: NP_003371
• MIM: 193060
• UniProt ID: P08670