Recombinant Human VIM, His-tagged

• Cat.No.: VIM-30158TH
• Product Overview: Recombinant full length Human Vimentin with N-terminal His tag, MWt 60kDa.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: This gene encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in this gene causes a dominant, pulverulent cataract.
• Conjugation: HIS
• Tissue specificity: Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitts lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines.
• Form: Liquid
• Purity: >95% by SDS-PAGE
• Storage buffer: Preservative: 150mM ImidazoleConstituents: 25% Glycerol, 50mM Sodium phosphate, 300mM Sodium chloride, 2mM DTT, 0.1mM PMSF, pH 7.0
• Storage: Shipped on dry ice. Upon delivery aliquot and store at -80oC. Avoid freeze / thaw cycles.
• Sequence Similarities: Belongs to the intermediate filament family.
• Full Length: Full L.

Gene Information

• Gene Name: VIM vimentin [ Homo sapiens ]
• Official Symbol: VIM
• Synonyms: VIM; vimentin
• Gene ID: 7431
• mRNA Refseq: NM_003380
• Protein Refseq: NP_003371
• Uniprot ID: P08670
• Chromosome Location: 10p13
• Pathway: Alpha6-Beta4 Integrin Signaling Pathway, organism-specific biosystem; Apoptosis, organism-specific biosystem; Apoptotic cleavage of cellular proteins, organism-specific biosystem; Apoptotic executionphase, organism-specific biosystem; Aurora B signaling, organism-specific biosystem
• Function: identical protein binding; protein C-terminus binding; protein binding; structural constituent of cytoskeleton