Recombinant Human VIM Full Length protein, His&Strep-tagged

• Cat.No.: VIM-17H
• Product Overview: Recombinant Human VIM Full Length protein(P08670) (Met1-Glu466) was expressed in Insect Cells with a C-terminal polyhistidine tag followed by an Strep Tag.

Specification

• Species: Human
• Source: Insect Cells
• Tag: His&Strep
• Protein Length: Met1-Glu466
• Tag: C-His-Strep
• Form: Lyophilized from sterile 40% acetonitrile, 0.1% TFA.
• Molecular Mass: The recombinant Human VIM consisting of 492 amino acids and has a calculated molecular mass of 56.59 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 62.7 kDa protein in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: ≥ 90 % as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: VIM vimentin [ Homo sapiens ]
• Official Symbol: VIM
• Synonyms: VIM; vimentin; FLJ36605
• Gene ID: 7431
• mRNA Refseq: NM_003380
• Protein Refseq: NP_003371
• UniProt ID: P08670