Recombinant Human VEGFC

• Cat.No.: VEGFC-157H
• Product Overview: Recombinant Human vascular endothelial growth factor C was expressed in modifiedhuman 293 cells.

Specification

• Species: Human
• Source: HEK293
• Tag: Non
• Description: Vascular endothelial growth factor C (VEGF-C), also known as Flt4 ligand (Flt4-L), vascular endothelial growth factor-related protein (VRP) and VEGF-2, is a member of the cysteine-knot growth factor superfamily and exhibits homology with VEGF-121 (32%) and PDGF (27%). However VEGF-C also possesses a 180 amino acid C-terminal cysteine-rich domain absent in other VEGFs. The major secreted form of VEGF-C is a disulfide linked antiparallel homodimeric protein containing 5 potential N-linked glycosylation sites and has a theoretical molecular weight of 44 kDa. VEGF-C is predominantly involved in angiogenesis, vasculogenesis, lymphangiogenesis as well as an inhibitor of dendritic cell maturation. VEGF-C also possesses both mitogenic and chemotactic activity w.r.t. endothelial cells and monocytes.
• Molecular Mass: VEGF-C secreted antiparallel homodimer migrates between 45 and 55 kDa due to post-translational modifications, in particular glycosylation. This compares with the unmodified homodimer that has a predicted molecular mass of 43.9 kDa. Individual chains of VEGF-C migrate as a band between 27 and 35 kDa in SDS-PAGE due to post-translational modifications, in particular glycosylation. This compares with the unmodified VEGF-C chains that have a predicted molecular mass of 21.6 and 22.2 kDa.
• PI: VEGF-C secreted antiparallel homodimer separates into a number of isoforms with a pI between 5.6 and 8.4 in 2D PAGE due to post-translational modifications, in particular glycosylation. This compares with the unmodified VEGF-C homodimer that has a predicted pI of 7.8.
• % Carbohydrate: VEGF-C consists of 15-40% from 1D gel of individual chains carbohydrate by weight.
• Glycosylation: VEGF-C contains N- and probably O-linked oligosaccharides.
• Purity: >95%, as determined by SDS-PAGE and visualized by silver stain.
• Formulation: When reconstituted in 0.5 ml sterile phosphate-buffered saline, the solution will contain 1% human serum albumin (HSA) and 10% trehalose.
• Reconstitution: It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial.
• Storage: Lyophilized products should be stored at 2 to 8°C. Following reconstitution short-term storage at 4°C is recommended, and longer-term storage of aliquots at -18 to -20°C.

Gene Information

• Gene Name: VEGFC vascular endothelial growth factor C [ Homo sapiens ]
• Synonyms: vascular endothelial growth factor C; VRP; Flt4-L; VEGFC; FLT4 ligand DHM; vascular endothelial growth factor-related protein; VEGF-C; Flt4 ligand; Vascular endothelial growth factor-related protein
• Gene ID: 7424
• mRNA Refseq: NM_005429
• Protein Refseq: NP_005420
• MIM: 601528
• UniProt ID: P49767
• Chromosome Location: 4q34.3
• Pathway: Bladder cancer; Cytokine-cytokine receptor interaction; Focal adhesion; Pancreatic cancer; Pathways in cancer; Renal cell carcinoma; mTOR signaling pathway; Hemostasis; Signaling by VEGF
• Function: growth factor activity; vascular endothelial growth factor receptor 3 binding