Recombinant Human VDAC1 lysate, Flag-tagged

• Cat.No.: VDAC1-732HCL
• Product Overview: Over-expression cells lysed in modified RIPA buffer.

Specification

• Species: Human
• Source: HEK293
• Tag: Flag
• Molecular Mass: 30.6 kDa
• Components: 1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer1 vial of 100 µg empty vector transfected control cell lysate in RIPA buffer1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromphenol blue, 100mM DTT)
• Preparation method: Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
• Recommended Usage: WB
• Storage Instruction: Lysate samples are stable for 12 months from date of receipt when stored at -20°C. Avoid repeated freeze-thaw cycles.Lysate samples can be diluted with 2xSDS Sample Buffer provided. After dilution, the protein sample should be aliquoted and stored at -20°C for long term storage. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.

Gene Information

• Gene Name: VDAC1 voltage-dependent anion channel 1 [ Homo sapiens ]
• Official Symbol: VDAC1
• Synonyms: VDAC1; voltage-dependent anion channel 1; voltage-dependent anion-selective channel protein 1; MGC111064; PORIN; porin 31HL; porin 31HM; plasmalemmal porin; outer mitochondrial membrane protein porin 1; VDAC-1
• Gene ID: 7416
• mRNA Refseq: NM_003374
• Protein Refseq: NP_003365
• MIM: 604492
• UniProt ID: P21796
• Chromosome Location: 5q31
• Pathway: Calcium signaling pathway, organism-specific biosystem; Calcium signaling pathway, conserved biosystem; HTLV-I infection, organism-specific biosystem; HTLV-I infection, conserved biosystem; Huntingtons disease, organism-specific biosystem; Huntingtons disease, conserved biosystem; Influenza A, organism-specific biosystem
• Function: porin activity; protein binding; voltage-gated anion channel activity; voltage-gated anion channel activity