Recombinant Human VCP protein, His & T7-tagged

• Cat.No.: VCP-7934H
• Product Overview: Recombinant Human VCP aa. (Gly125~Ile371 (Accession # P55072)) fused with N-terminal His & T7 tag was produced in E. coli cells.

Specification

• Species: Human
• Source: E.coli
• Tag: His&T7
• Protein Length: Gly125~Ile371
• Description: The protein encoded by this gene is a member of a family that includes putative ATP-binding proteins involved in vesicle transport and fusion, 26S proteasome function, and assembly of peroxisomes. This protein, as a structural protein, is associated with clathrin, and heat-shock protein Hsc70, to form a complex. It has been implicated in a number of cellular events that are regulated during mitosis, including homotypic membrane fusion, spindle pole body function, and ubiquitin-dependent protein degradation.
• Form: Freeze-dried powder
• Molecular Mass: 36kDa as determined by SDS-PAGE reducing conditions.
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 5.9.
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage Buffer: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

Gene Information

• Gene Name: VCP valosin containing protein [ Homo sapiens (human) ]
• Official Symbol: VCP
• Synonyms: VCP; valosin containing protein; p97; TERA; ALS14; CDC48; CMT2Y; IBMPFD; HEL-220; IBMPFD1; HEL-S-70; transitional endoplasmic reticulum ATPase; 15S Mg(2+)-ATPase p97 subunit; TER ATPase; epididymis luminal protein 220; epididymis secretory protein Li 70; yeast Cdc48p homolog
• Gene ID: 7415
• mRNA Refseq: NM_007126.3
• Protein Refseq: NP_009057.1
• UniProt ID: P55072