Recombinant Human VAPB, His-tagged

• Cat.No.: VAPB-2875H
• Product Overview: Recombinant human VAPB protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: VAPB, also known as vesicle-associated membrane protein (VAMP)-associated protein B, is a type IV transmembrane protein and member of the VAP family of proteins. This protein may play a role in vesicle trafficking. It is found in plasma and intracellular vesicle membranes as a homodimer and heterodimer with VAPA, and interacts with VAMP1 and VAMP2. Defects in VAPB are a cause of amyotrophic lateral sclerosis type 8 and spinal muscular atrophy autosomal dominant Finkel type.
• Sequence: MGSSHHHHHH SSGLVPRGSH MAKVEQVLSL EPQHELKFRG PFTDVVTTNL KLGNPTDRNV CFKVKTTAPR RYCVRPNSGI IDAGASINVS VMLQPFDYDP NEKSKHKFMV QSMFAPTDTS DMEAVWKEAK PEDLMDSKLR CVFELPAEND KPHDVEINKI ISTTASKTET PIVSKSLSSS LDDTEVKKVM EECKRLQGEV QRLREENKQF KEEDGLRMRK TVQSNSPISA LAPTGKEEGL ST.
• Purity: > 90% by SDS – PAGE.
• MW: 27.1 kDa (242aa) confirmed by MALDI-TOF.
• Form: Liquid. 20mM Tris-HCl buffer (pH8.0) containing 10% glycerol, 2mM DTT, 50mM NaCl, 0.1mM PMSF.
• Concentration: 1mg/ml (determined by Bradford assay).
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: VAPB VAMP (vesicle-associated membrane protein)-associated protein B and C [ Homo sapiens ]
• Synonyms: VAPB; VAMP (vesicle-associated membrane protein)-associated protein B and C; RP5-1018E9.1, ALS8, VAMP-B, VAP-B; VAMP-associated 33 kDa protein; vesicle-associated membrane protein-associated protein B/C
• Gene ID: 9217
• mRNA Refseq: NM_001195677
• Protein Refseq: NP_001182606
• MIM: 605704
• UniProt ID: O95292
• Chromosome Location: 20q13.33
• Pathway: Metabolism of lipids and lipoproteins
• Function: beta-tubulin binding; enzyme binding; protein heterodimerization activity; structural molecule activity