Recombinant Human V-Raf Murine Sarcoma Viral Oncogene Homolog B1, GST-tagged

• Cat.No.: BRAF-994H
• Product Overview: Recombinant human BRAF (416-end; V599E) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Specification

• Species: Human
• Source: Sf9 Cells
• Tag: GST
• Protein Length: 416-end a.a.
• Description: BRAF is a member of the RAF family that is activated by members of the Ras family upon growth factor-induced stimulation. Active Ras can induce heterodimerization of cRaf and BRAF and this may explain the observed cooperativity of cRaf and BRaf in cells responding to growth factor signals. Activating mutations in the BRAF gene are present in a large percentage of human malignant melanomas and in a proportion of colon cancers. The vast majority of these mutations result in a valine to glutamic acid change at residue 599 within the activation segment of B-RAF.
• Applications: Kinase Assay; Western Blot
• Molecular Weight: 63 kDa
• Expression System: Sf9 insect cells using baculovirus
• Form: Recombinant protein stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25 % glycerol.
• Specific Activity: 98 nmol/min/mg
• Purity: > 80 %
• Concentration: 0.1 ug/ul
• Sequences: 416-end
• Storage: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Pathways: Acute myeloid leukemia; Bladder cancer; Chemokine signaling pathway; Chronic myeloid leukemia; Colorectal cancer; Endometrial cancer; ErbB signaling pathway; Focal adhesion; Glioma; Insulin signaling pathway; Long-term depression; Long-term potentiation; MAPK signaling pathway; Melanoma; Natural killer cell mediated cytotoxicity; Neurotrophin signaling pathway

Gene Information

• Gene Name: BRAF v-raf murine sarcoma viral oncogene homolog B1 [ Homo sapiens ]
• Official Symbol: BRAF
• Synonyms: BRAF; v-raf murine sarcoma viral oncogene homolog B1; NS7; BRAF1; RAFB1; B-RAF1; FLJ95109; MGC126806; MGC138284; serine/threonine-protein kinase B-raf; p94; 94 kDa B-raf protein; proto-oncogene B-Raf; murine sarcoma viral (v-raf) oncogene homolog B1; B-Raf proto-oncogene serine/threonine-protein kinase (p94); Serine/threonine-protein kinase B-raf; Proto-oncogene B-Raf; EC 2.7.11.1; OTTHUMP00000212184
• Gene ID: 673
• mRNA Refseq: NM_004333
• Protein Refseq: NP_004324
• MIM: 164757
• UniProt ID: P15056
• Chromosome Location: 7q34
• Function: mitogen-activated protein kinase kinase binding; MAP kinase kinase kinase activity; protein heterodimerization activity; protein serine/threonine kinase activity; protein tyrosine kinase activity; receptor signaling protein activity; ATP binding; metal ion binding; nucleotide binding; protein binding; transferase activity; zinc ion binding