Recombinant Human BRAF protein, GST-tagged

• Cat.No.: BRAF-254H
• Product Overview: Recombinant Human BRAF protein(P15056)(E433-R726), fused with N-terminal GST tag, was expressed in Sf9 insect cell.

Specification

• Species: Human
• Source: Sf9 Cells
• Tag: GST
• Protein Length: E433-R726
• Tag: N-GST
• Form: Liquid in 50 mM Tris, 150 mM NaCl, 20 mM Reduced glutathione, 10% glycerol, 0.05% Brij35, pH7.5.
• Bio-activity: The BRAF activity was detected using HTRF technology. The reaction was performed by incubating the BRAF protein, ATP and substrate at 25°C for 60 min, adding the beads at 25°C for 60 min, then reading ratio 665/620 with BMG.
• Molecular Mass: 60.3 kDa
• Purity: > 35% by SDS-PAGE
• Storage: Stable for 12 months upon stored at -80°C from the date of receipt. And avoid repeated freeze-thaws cycles.
• Concentration: 0.18 mg/mL by OD280.
• Reconstitution: Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: BRAF v-raf murine sarcoma viral oncogene homolog B1 [ Homo sapiens ]
• Official Symbol: BRAF
• Synonyms: BRAF; v-raf murine sarcoma viral oncogene homolog B1; serine/threonine-protein kinase B-raf; BRAF1; p94; 94 kDa B-raf protein; proto-oncogene B-Raf; murine sarcoma viral (v-raf) oncogene homolog B1; B-Raf proto-oncogene serine/threonine-protein kinase (p94); NS7; RAFB1; B-RAF1; FLJ95109; MGC126806; MGC138284
• Gene ID: 673
• mRNA Refseq: NM_004333
• Protein Refseq: NP_004324
• MIM: 164757
• UniProt ID: P15056