Recombinant Human USP46, His-tagged

• Cat.No.: USP46-5057H
• Product Overview: Recombinant human USP46 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Specification

• Description: Modification of cellular proteins by ubiquitin is an essential regulatory mechanism controlled by the coordinated action of multiple ubiquitin-conjugating and deubiquitinating enzymes. USP46 belongs to a large family of cysteine proteases that function as deubiquitinating enzymes.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT
• Molecular Mass: 44.8kDa (389aa) confirmed by MALDI-TOF
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSMTVRNIA SICNMGTNAS ALEKDIGPEQ FPINEHYFGL VNFGNTCYCN SVLQALYFCR PFRENVLAYK AQQKKKENLL TCLADLFHSI ATQKKKVGVI PPKKFISRLR KENDLFDNYM QQDAHEFLNY LLNTIADILQ EEKKQEKQNG KLKNGNMNEP AENNKPELTW VHEIFQGTLT NETRCLNCET VSSKDEDFLD LSVDVEQNTS ITHCLRDFSN TETLCSEQKY YCETCCSKQE AQKRMRVKKL PMILALHLKR FKYMEQLHRY TKLSYRVVFP LELRLFNTSS DAVNLDRMYD LVAVVVHCGS GPNRGHYITI VKSHGFWLLF DDDIVEKIDA QAIEEFYGLT SDISKNSESG YILFYQSRE
• Purity: >90% by SDS - PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at +4centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20centigrade or -70centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 0.25 mg/ml (determined by BRADFORD assay)

Gene Information

• Gene Name: USP46 ubiquitin specific peptidase 46 [ Homo sapiens ]
• Official Symbol: USP46
• Synonyms: USP46; ubiquitin specific peptidase 46; ubiquitin specific protease 46; ubiquitin carboxyl-terminal hydrolase 46; FLJ12552; ubiquitin thioesterase 46; deubiquitinating enzyme 46; ubiquitin thiolesterase 46; ubiquitin-specific-processing protease 46; FLJ11850; FLJ14283; FLJ39393
• Gene ID: 64854
• mRNA Refseq: NM_001134223
• Protein Refseq: NP_001127695
• MIM: 612849
• UniProt ID: P62068
• Chromosome Location: 4q12
• Function: cysteine-type peptidase activity; peptidase activity; protein binding; ubiquitin thiolesterase activity; ubiquitin-specific protease activity