Recombinant Human USP40 overexpression lysate, Myc/DDK-tagged

• Cat.No.: USP40-1001HCL
• Product Overview: Transient overexpression lysate of ubiquitin specific peptidase 40 (USP40) with a C-Myc/DDK tag was expressed in HEK293T.
  HEK293T cells in 10-cm dishes were transiently transfected with cDNA plasmid. Transfected cells were cultured for 48 hrs before collection. The cells were lysed in modified RIPA buffer (25 mM Tris-HCl pH 7.6, 150 mM NaCl, 1% NP-40, 1 mM EDTA, 1 x Proteinase inhibitor cocktail mix, 1 mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20 centigrade before shipping.
  Components:
  1 vial of 100 μg gene specific transient over-expression cell lysate in RIPA buffer
  1 vial of 100 μg empty vector transfected control cell lysate in RIPA buffer
  1 vial of 250 μL 2 x SDS Sample Buffer (4% SDS, 125 mM Tris-HCl pH 6.8, 10% Glycerol, 0.002% Bromphenol blue, 100 mM DTT)

Specification

• Description: Modification of cellular proteins by ubiquitin is an essential regulatory mechanism controlled by the coordinated action of multiple ubiquitin-conjugating and deubiquitinating enzymes. USP40 belongs to a large family of cysteine proteases that function as deubiquitinating enzymes.
• Source: HEK293T
• Species: Human
• Tag: Myc&DDK
• Molecular Mass: 141.4 kDa
• Storage: Upon receiving, store the sample at -80 centigrade. Also after dilution, the protein sample should be aliquoted and stored at -80 centigrade for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 2 x SDS Sample Buffer provided. Lysate samples are stable for 12 months from the date of receipt when stored at -80 centigrade.
• Shipping: Dry ice

Gene Information

• Gene Name: USP40 ubiquitin specific peptidase 40 [ Homo sapiens (human) ]
• Official Symbol: USP40
• Synonyms: USP40; ubiquitin specific peptidase 40; ubiquitin carboxyl-terminal hydrolase 40; deubiquitinating enzyme 40; ubiquitin specific protease 40; ubiquitin thioesterase 40; ubiquitin thiolesterase 40; ubiquitin-specific-processing protease 40; EC 3.4.19.12
• Gene ID: 55230
• mRNA Refseq: NM_018218
• Protein Refseq: NP_060688
• MIM: 610570
• UniProt ID: Q9NVE5