Recombinant Human USP1/UAF1 Complex Protein, His-Tagged

• Cat.No.: USP1-161H
• Product Overview: Spodoptera frugiperda, Sf 21 (baculovirus)-derived human USP1/UAF1 Complex protein. Contains a C-terminal 6-His tag

Specification

• Species: Human
• Source: Sf21 Cells
• Tag: His
• Description: USP1 (Ubiquitin-Specific-processing Protease 1, also known as Ubiquitin carboxyl-terminal hydrolase 1) is a deubiquitinating enzyme of the C19 peptidase family and functions as a negative regulator of the Fanconi Anemia pathway. Reported substrates of USP1 include monoubiquitinated FANCD2 and monoubiquitinated PCNA. USP1 plays important roles in DNA damage responses and cancer-related processes, and inhibiting the function of this deubiquitinase sensitizes some cancer cells to chemotherapy. By itself, USP1 is nearly completely inactive and requires a protein binding partner, UAF1 (USP1-Associated Factor 1, also known as WD Repeat-containing protein 48 or WDR48) to stimulate its deubiquitinase activity. In addition to the naturally occurring substates listed above, the USP1/UAF1 complex has been demonstrated to cleave K6, K33, and K63-linked polyubiquitin chains. Recombinant USP1 protein contains a C-terminal 6-His tag, and glycine-to-alanine substitutions at positions 670 and 671 (UniProt O94782 numbering). Recombinant UAF1 protein also contains a C-terminal 6-His tag. The assembled complex contains 25 µg each of USP1 and UAF1, resulting in a 1:1.2 stoichiometry.
• Molecular Mass: 89 kDa (USP1), 77 kDa (UAF1)
• Purity: >80%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain
• Applications: Bioactivity
• Usage: Recombinant Human USP1/UAF1 complex is a Ubiquitin-specific deconjugating enzyme heterodimer. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human USP1/UAF1 complex concentration of 0.01-0.5 μM.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -70 °C as supplied.
  3 months, -70 °C under sterile conditions after opening.
• Storage Buffer: X mg/ml total protein including USP1 (X µM) and UAF1(X µM) in 50 mM HEPES, pH 7.5, 100 mM NaCl, 10% glycerol, 1 mM TCEP
• Shipping: The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.