Name: Recombinant Human USH1G 293 Cell Lysate
SKU: USH1G-477HCL

Species :	Human
Source :	HEK293
Tag :	Non
Description :	Antigen standard for Usher syndrome 1G (autosomal recessive) (USH1G) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Components :	This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size :	0.1 mg
Storage Instruction :	Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications :	ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Name	USH1G Usher syndrome 1G (autosomal recessive) [ Homo sapiens ]
Official Symbol	USH1G
Synonyms	USH1G; Usher syndrome 1G (autosomal recessive); Usher syndrome type-1G protein; ANKS4A; FLJ33924; Sans; scaffold protein containing ankyrin repeats and SAM domain; SANS;
Gene ID	124590
mRNA Refseq	NM_173477
Protein Refseq	NP_775748
MIM	607696
UniProt ID	Q495M9
Chromosome Location	17q25.2