Recombinant human USF1, His-tagged

• Cat.No.: USF1-2447H
• Product Overview: Recombinant human USF1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Specification

• Description: USF1 is a member of the basic helix-loop-helix leucine zipper family, and can function as a cellular transcription factor. USF1 can activate transcription through pyrimidine-rich initiator (Inr) elements and E-box motifs. This gene has been linked to familial combined hyperlipidemia (FCHL). Alternative splicing of this gene results in multiple transcript variants. A related pseudogene has been defined on chromosome 21.
• Source: E.coli
• Species: Human
• Tag: His
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol, 1mM DTT
• Molecular Mass: 35.9kDa (333aa), confirmed by MALDI-TOF
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSMKGQQKT AETEEGTVQI QEGAVATGED PTSVAIASIQ SAATFPDPNV KYVFRTENGG QVMYRVIQVS EGQLDGQTEG TGAISGYPAT QSMTQAVIQG AFTSDDAVDT EGTAAETHYT YFPSTAVGDG AGGTTSGSTA AVVTTQGSEA LLGQATPPGT GQFFVMMSPQ EVLQGGSQRS IAPRTHPYSP KSEAPRTTRD EKRRAQHNEV ERRRRDKINN WIVQLSKIIP DCSMESTKSG QSKGGILSKA CDYIQELRQS NHRLSEELQG LDQLQLDNDV LRQQVEDLKN KNLLLRAQLR HHGLEVVIKN DSN
• Purity: >90% by SDS - PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.25 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: USF1 upstream transcription factor 1 [ Homo sapiens ]
• Official Symbol: USF1
• Synonyms: USF1; upstream transcription factor 1; upstream stimulatory factor 1; bHLHb11; MLTFI; UEF; major late transcription factor 1; class B basic helix-loop-helix protein 11; FCHL; MLTF; FCHL1; HYPLIP1
• Gene ID: 7391
• mRNA Refseq: NM_007122
• Protein Refseq: NP_009053
• MIM: 191523
• UniProt ID: P22415
• Chromosome Location: 1q22-q23
• Pathway: Signaling mediated by p38-alpha and p38-beta, organism-specific biosystem
• Function: bHLH transcription factor binding; double-stranded DNA binding; enzyme binding; histone deacetylase binding; protein binding; protein heterodimerization activity; protein homodimerization activity; protein homodimerization activity; protein kinase binding