Recombinant Human UPRT protein, His-tagged

• Cat.No.: UPRT-2309H
• Product Overview: Recombinant Human UPRT(1-309aa) fused with His tag at N-terminal was expressed in E. coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 1-309 a.a.
• Description: uPRT is a uracil phosphoribosyltransferase, which catalyzes the conversion of uracil and 5-phosphoribosyl-1-R-diphosphate to uridine monophosphate (uMP). This reaction is an important part of nucleotide metabolism, specifically the pyrimidine salvage pathway. The enzyme localizes to the nucleus and cytoplasm. The protein is a potential target for rational design of drugs to treat parasitic infections and cancer.
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol, 1mM DTT
• Molecular Mass: 36.2 kDa (332aa) confirmed by MALDI-TOF
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSMATELQC PDSMPCHNQQ VNSASTPSPE QLRPGDLILD HAGGNRASRA KVILLTGYAH SSLPAELDSGACGGSSLNSE GNSGSGDSSS YDAPAGNSFL EDCELSRQIG AQLKLLPMND QIRELQTIIR DKTASRGDFM FSADRLIRLV VEEGLNQLPYKECMVTTPTG YKYEGVKFEK GNCGVSIMRS GEAMEQGLRD CCRSIRIGKI LIQSDEETQR AKVYYAKFPP DIYRRKVLLM YPILSTGNTVIEAVKVLIEH GVQPSVIILL SLFSTPHGAK SIIQEFPEIT ILTTEVHPVA PTHFGQKYFG TD
• Purity: > 90% by SDS - PAGE
• Storage: Can be stored at +4 centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20 centigrade or -70 centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 0.5 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: UPRT uracil phosphoribosyltransferase (FUR1) homolog (S. cerevisiae) [ Homo sapiens ]
• Official Symbol: UPRT
• Synonyms: UPRT; uracil phosphoribosyltransferase (FUR1) homolog (S. cerevisiae); uracil phosphoribosyltransferase homolog; DKFZp781E1243; FUR1; MGC23937; RP11 311P8.3; UPRTase; UMP pyrophosphorylase; UPP; RP11-311P8.3
• Gene ID: 139596
• mRNA Refseq: NM_145052
• Protein Refseq: NP_659489
• MIM: 300656
• UniProt ID: Q96BW1
• Chromosome Location: Xq13.3
• Pathway: Metabolic pathways, organism-specific biosystem; Pyrimidine metabolism, organism-specific biosystem; Pyrimidine metabolism, conserved biosystem