Recombinant Human UPF1 protein, His-tagged

• Cat.No.: UPF1-3089H
• Product Overview: Recombinant Human UPF1 protein(310-607 aa), fused to His tag, was expressed in E. coli.

Specification

• Source: E. coli
• Species: Human
• Tag: His
• Form: The purified protein was Lyophilized from sterile PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH7.4). 5 % trehalose and 5 % mannitol are added as protectant before lyophilization. The elution buffer contain 300mM imidazole.
• Protein length: 310-607 aa
• AA Sequence: VKLEADYDKKLKESQTQDNITVRWDLGLNKKRIAYFTLPKTDSDMRLMQGDEICLRYKGDLAPLWKGIGHVIKVPDNYGDEIAIELRSSVGAPVEVTHNFQVDFVWKSTSFDRMQSALKTFAVDETSVSGYIYHKLLGHEVEDVIIKCQLPKRFTAQGLPDLNHSQVYAVKTVLQRPLSLIQGPPGTGKTVTSATIVYHLARQGNGPVLVCAPSNIAVDQLTEKIHQTGLKVVRLCAKSREAIDSPVSFLALHNQIRNMDSMPELQKLQQLKDETGELSSADEKRYRALKRTAERELL
• Purity: 95%, by SDS-PAGE with Coomassie Brilliant Blue staining.
• Storage: Short-term storage: Store at 2-8°C for (1-2 weeks).
  Long-term storage: Aliquot and store at -20°C to -80°C for up to 3 months, buffer containing 50% glycerol is recommended for reconstitution. Avoid repeat freeze-thaw cycles.
• Reconstitution: Reconstitute at 0.25 µg/μl in 200 μl sterile water for short-term storage.
  Reconstitution with 200 μl 50% glycerol solution is recommended for longer term storage.

Gene Information

• Gene Name: UPF1 UPF1 regulator of nonsense transcripts homolog (yeast) [ Homo sapiens ]
• Official Symbol: UPF1
• Synonyms: UPF1; UPF1 regulator of nonsense transcripts homolog (yeast); regulator of nonsense transcripts 1 , RENT1; regulator of nonsense transcripts 1; HUPF1; KIAA0221; NORF1; pNORF1; smg 2; smg 2 homolog; nonsense mediated mRNA decay factor (C. elegans); UP Frameshift 1; delta helicase; yeast Upf1p homolog; ATP-dependent helicase RENT1; nonsense mRNA reducing factor 1; up-frameshift mutation 1 homolog; up-frameshift suppressor 1 homolog; smg-2 homolog, nonsense mediated mRNA decay factor; RENT1; smg-2; FLJ43809; FLJ46894
• Gene ID: 5976
• mRNA Refseq: NM_002911
• Protein Refseq: NP_002902
• MIM: 601430
• UniProt ID: Q92900