Recombinant Human Unc-51-Like Kinase 2 (C. elegans), GST-tagged

• Cat.No.: ULK2-1115H
• Product Overview: Recombinant human ULK2 (1-478) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Specification

• Species: Human
• Source: Sf10 Insect Cell
• Tag: GST
• Protein Length: 1-478 a.a.
• Description: ULK2 is a serine/threonine protein kinase that plays critical role during initial stages of autophagy which is a vital response to nutrient starvation. Mammalian Atg13 binds ULK2 and mediates the interaction of the ULK protein with FIP200. The binding of Atg13 stabilizes and activates ULK and facilitates the phosphorylation of FIP200 by ULK. The ULK-Atg13-FIP200 complex is a direct target of mTOR and important regulator of autophagy in response to mTORsignalling. Yeast 2-hybrid assays showed the C-terminus of ULK2 binds the C-terminus of SynGAP, a negative regulator of Ras that is associated with neural development.
• Applications: Kinase Assay; Western Blot
• Molecular Weight: 98kDa
• Expression System: Sf9 insect cells using baculovirus
• Form: Recombinant protein stored in 50mM Tris-HCI, pH 7.5, 150mM NaCl, 0.25 mM DTT, 0.1mM EGTA, 0.1mM EDTA,0.1mM PMSF, 25% glycerol.
• Specific Activity: 102nmol/min/mg
• Purity: >80%
• Concentration: 0.1 ug/ul
• Sequences: 1-478
• Storage: Store product at -70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles. Product shipped on dry ice.

Gene Information

• Gene Name: ULK2 unc-51-like kinase 2 (C. elegans) [ Homo sapiens ]
• Official Symbol: ULK2
• Synonyms: ULK2; unc-51-like kinase 2 (C. elegans); Unc51.2; KIAA0623; ULK2; unc-51-like kinase 2; EC 2.7.11.1; EC 2.7.11.1; OTTHUMP00000065847; unc-51 (C. elegans)-like kinase 2; ATG1B; serine/threonine-protein kinase ULK2; OTTHUMP00000065848
• Gene ID: 9706
• mRNA Refseq: NM_014683
• Protein Refseq: NP_055498
• MIM: 608650
• UniProt ID: Q8IYT8
• Chromosome Location: 17p11.2
• Pathway: Regulation of autophagy; mTOR signaling pathway
• Function: ATP binding; nucleotide binding; protein binding; protein serine/threonine kinase activity; transferase activity