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Recombinant Human UHMK1 293 Cell Lysate

Cat.No. : UHMK1-509HCL
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Description : Antigen standard for U2AF homology motif (UHM) kinase 1 (UHMK1) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Tag : Non
Gene Name : UHMK1 U2AF homology motif (UHM) kinase 1 [ Homo sapiens ]
Official Symbol : UHMK1
Synonyms : UHMK1; U2AF homology motif (UHM) kinase 1; serine/threonine-protein kinase Kist; KIS; Kist; KIS protein kinase; kinase interacting with leukemia-associated gene (stathmin); KIST; FLJ23015; DKFZp434C1613;
Gene ID : 127933
mRNA Refseq : NM_001184763
Protein Refseq : NP_001171692
MIM : 608849
UniProt ID : Q8TAS1
Chromosome Location : 1q23.1

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