Recombinant Human UFD1L, His-tagged

• Cat.No.: UFD1L-15885H
• Product Overview: Recombinant human UFD1L protein, fused to His tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Specification

• Description: UFD1L forms a complex with two other proteins, nuclear protein localization-4 and valosin-containing protein, and this complex is necessary for the degradation of ubiquitinated proteins. In addition, this complex controls the disassembly of the mitotic spindle and the formation of a closed nuclear envelope after mitosis. Mutations in this gene have been associated with Catch 22 syndrome as well as cardiac and craniofacial defects. Alternative splicing results in multiple transcript variants encoding different isoforms. A related pseudogene has been identified on chromosome 18.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 30% glycerol, 1mM DTT
• Molecular Mass: 36.9 kDa (330aa), confirmed by MALDI-TOF(Molecular size on SDS-PAGE will appear higher)
• Protein length: 1-307aa
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSMFSFNMF DHPIPRVFQN RFSTQYRCFS VSMLAGPNDR SDVEKGGKII MPPSALDQLS RLNITYPMLF KLTNKNSDRM THCGVLEFVA DEGICYLPHW MMQNLLLEEG GLVQVESVNL QVATYSKFQP QSPDFLDITN PKAVLENALR NFACLTTGDV IAINYNEKIY ELRVMETKPD KAVSIIECDM NVDFDAPLGY KEPERQVQHE ESTEGEADHS GYAGELGFRA FSGSGNRLDG KKKGVEPSPS PIKPGDIKRG IPNYEFKLGK ITFIRNSRPL VKKVEEDEAG GRFVAFSGEG QSLRKKGRKP
• Purity: >85% by SDS - PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at 4°C short term. For long term storage, aliquot and store at at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.25mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: UFD1L ubiquitin fusion degradation 1 like (yeast) [ Homo sapiens ]
• Official Symbol: UFD1L
• Synonyms: UFD1L; ubiquitin fusion degradation 1 like (yeast); ubiquitin fusion degradation 1 like; ubiquitin fusion degradation protein 1 homolog; UFD1; UB fusion protein 1
• Gene ID: 7353
• mRNA Refseq: NM_005659
• Protein Refseq: NP_005650
• MIM: 601754
• UniProt ID: Q92890
• Chromosome Location: 22q11.2
• Pathway: HRD1/SEL1 ERAD complex, organism-specific biosystem; HRD1/SEL1 ERAD complex, conserved biosystem; Protein processing in endoplasmic reticulum, organism-specific biosystem; Protein processing in endoplasmic reticulum, conserved biosystem; p97-Ufd1-Npl4 complex, organism-specific biosystem
• Function: protein binding; ubiquitin-specific protease activity