Recombinant Human UCP1 protein, His-tagged

• Cat.No.: UCP1-2916H
• Product Overview: Recombinant Human UCP1 protein(1-307 aa), fused to His tag, was expressed in E. coli.

Specification

• Source: E. coli
• Species: Human
• Tag: His
• Form: The purified protein was Lyophilized from sterile PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH7.4). 5 % trehalose and 5 % mannitol are added as protectant before lyophilization. The elution buffer contain 300mM imidazole.
• Protein length: 1-307 aa
• AA Sequence: MGGLTASDVHPTLGVQLFSAGIAAC LADVITFPLDTAKVRLQVQGECPTS SVIRYKGVLGTITAVVKTEGRMKLY SGLPAGLQRQISSASLRIGLYDTVQ EFLTAGKETAPSLGSKILAGLTTGG VAVFIGQPTEVVKVRLQAQSHLHGI KPRYTGTYNAYRIIATTEGLTGLWK GTTPNLMRSVIINCTELVTYDLMKE AFVKNNILADDVPCHLVSALIAGFC ATAMSSPVDVVKTRFINSPPGQYKS VPNCAMKVFTNEGPTAFFKGLVPSF LRLGSWNVIMFVCFEQLKRELSKSR QTMDCAT
• Purity: 80%, by SDS-PAGE with Coomassie Brilliant Blue staining.
• Storage: Short-term storage: Store at 2-8°C for (1-2 weeks).
  Long-term storage: Aliquot and store at -20°C to -80°C for up to 3 months, buffer containing 50% glycerol is recommended for reconstitution. Avoid repeat freeze-thaw cycles.
• Reconstitution: Reconstitute at 0.25 µg/μl in 200 μl sterile water for short-term storage.
  Reconstitution with 200 μl 50% glycerol solution is recommended for longer term storage.

Gene Information

• Gene Name: UCP1 uncoupling protein 1 (mitochondrial, proton carrier) [ Homo sapiens ]
• Official Symbol: UCP1
• Synonyms: UCP1; uncoupling protein 1 (mitochondrial, proton carrier); UCP; mitochondrial brown fat uncoupling protein 1; SLC25A7; thermogenin; solute carrier family 25 member 7
• Gene ID: 7350
• mRNA Refseq: NM_021833
• Protein Refseq: NP_068605
• MIM: 113730
• UniProt ID: P25874

Reviews

05/14/2020

Standardized usage protocols of protein reagents facilitate data sharing and comparison with other laboratories.

06/22/2019

The application process of protein reagents is convenient, requiring minimal complex sample handling steps.

03/11/2017

Protein reagents possess high purity, guaranteeing reliable results without interference from impurities.