Recombinant Human UCHL5, His-tagged

• Cat.No.: UCHL5-1519H
• Product Overview: Recombinant human UCHL5 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques (1-329aa).

Specification

• Cat. No.: UCHL5-1519H
• Description: Ubiquitin carboxyl-terminal hydrolase isozyme L5 (UCHL5) belongs to the peptidase C12 family. This protein is protease that specifically cleaves "Lys-48"-linked polyubiquitin chains, and deubiquitinating enzyme associated with the 19S regulatory subunit of the 26S proteasome.
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 5mM DTT, 30% glycerol, 200mM NaCl, 0.1mM PMSF, 2mM EDTA
• Molecular Weight: 39.7kDa (349aa), confirmed by MALDI-TOF
• Purity: > 90% by SDS - PAGE
• Concentration: 0.5mg/ml (determined by Bradford assay)
• Biological activity: Specific activity: > 500 pmole/min/ug. Measured by the hydrolysis of Ubiquitin-AMC at pH 8.0, at 37°C.
• Activity Assay: 1. Prepare a 100ul of recombinant UCH-L5 protein with various concentrations (0.48ng, 0.9ng) in assay buffer and equilibrate to 37°C for 10 minutes. (Assay buffer: 50mM Tris-HCl, 0.5 mM EDTA, 1 mM DTT, 0.1 mg/ml Ovalbumin, pH 8.0.) 2. Add 50ul of 1uM Ubiquitin-AMC. 3. Read at excitation wavelengths 355nm and emission 460nm for 5 minutes. - Ubiquitin-AMC - 96 Well Polystyrene Microplate, black - Fluorescent plate reader
• Sequences of amino acids: MGSSHHHHHH SSGLVPRGSH MTGNAGEWCL MESDPGVFTE LIKGFGCRGA QVEEIWSLEP ENFEKLKPVH GLIFLFKWQP GEEPAGSVVQ DSRLDTIFFA KQVINNACAT QAIVSVLLNC THQDVHLGET LSEFKEFSQS FDAAMKGLAL SNSDVIRQVH NSFARQQMFE FDTKTSAKEE DAFHFVSYVP VNGRLYELDG LREGPIDLGA CNQDDWISAV RPVIEKRIQK YSEGEIRFNL MAIVSDRKMI YEQKIAELQR QLAEEEPMDT DQGNSMLSAI QSEVAKNQML IEEEVQKLKR YKIENIRRKH NYLPFIMELL KTLAEHQQLI PLVEKAKEKQ NAKKAQETK
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: UCHL5 ubiquitin carboxyl-terminal hydrolase L5 [ Homo sapiens ]
• Official Symbol: UCHL5
• Synonyms: UCHL5; UCH37; CGI-70; INO80R; AD-019; UCH-L5; ubiquitin carboxyl-terminal hydrolase L5; ubiquitin carboxyl-terminal hydrolase isozyme L5; OTTHUMP00000033565; OTTHUMP00000033566; OTTHUMP00000033567; OTTHUMP00000033568; OTTHUMP00000033570; OTTHUMP00000033571; OTTHUMP00000033754; INO80 complex subunit R; ubiquitin thioesterase L5; ubiquitin C-terminal hydrolase UCH37; ubiquitin carboxyl-terminal esterase L5; EC 3.4.19.12
• Gene ID: 51377
• mRNA Refseq: NM_015984
• Protein Refseq: NP_057068
• MIM: 610667
• UniProt ID: Q9Y5K5
• Chromosome Location: 1q32
• Function: cysteine-type peptidase activity; endopeptidase inhibitor activity; omega peptidase activity; peptidase activity; proteasome binding; protein binding; ubiquitin thiolesterase activity; ubiquitin-specific protease activity

Reviews

02/25/2023

The reproducibility is very good and I get consistent results every time, which makes me feel more confident in the results.

09/07/2020

The activity of UCHL5 is maintained for a long time, which ensures the stability of the experimental results.

03/25/2020

Excellent performance in Western blot, avoiding interference problems in experiments.

Q&As

How is the expression level of UCHL5 protein regulated?

The expression level of UCHL5 protein may be regulated by a variety of factors, including gene transcription, translation, post-transcriptional modification, etc. Studying these regulatory mechanisms can help to understand the expression patterns of UCHL5 protein in organisms and its effects on biological processes.

What is the expression pattern of UCHL5 protein?

UCHL5 protein may be highly expressed in specific tissues and cells and low in other tissues and cells. Its expression pattern can be influenced by a variety of factors, including gene regulation, transcription factors, and/or signal transduction pathways.

What are the effects of mutations or modifications in the UCHL5 protein on organisms?

Mutations or modifications of the UCHL5 protein may affect its function and thus affect the organism. The specific effects may vary depending on the type and location of the mutation or modification and may involve various biological processes, such as growth and development, immune response, etc.

What biological processes does UCHL5 protein play a role in?

This protein may be involved in a variety of biological processes, including protein quality control, cell cycle regulation, DNA repair, and signal transduction. These processes often require the involvement of UPS to achieve protein degradation and regulation.

How does UCHL5 protein interact with other proteins?

UCHL5 proteins may interact with other proteins to fulfill their functions. These interactions can involve various types of protein-protein interactions like electrostatic interactions, hydrogen bonding, etc. Understanding these interactions can help unravel the mechanism of action of the UCHL5 protein in cells.

What is the function of UCHL5 protein?

The main function of UCHL5 protein may be as a component of the ubiquitin-proteasome system (UPS), which is involved in the regulation of protein quality control, cell cycle regulation, DNA repair, and signal transduction. It may perform these functions by hydrolyzing specific substrates.