Recombinant Human Ubiquitin-Like Modifier Activating Enzyme 5, His-tagged

• Cat.No.: UBA5-1958H
• Product Overview: Recombinant humanUBA5 protein, fused to His-tag at N-terminus, was expressed in E. coli andpurified by using conventional chromatography techniques.

Specification

• Cat. No.: UBA5-1958H
• Description: UBA5(ubiquitin-like modifier activating enzyme 5) belongs to theubiquitin-activating E1 family and UBA5 subfamily. Ubiquitin andubiquitin-like proteins are known to be covalently conjugated to a variety ofcellular substrates via a three-step enzymatic pathway. Theubiquitin-activating enzyme (E1) plays a key role in the first step ofubiquitination pathway to activate ubiquitin or ubiquitin-like proteins. UBA5had been proved to activate an ubiquitin-like protein, ubiquitin-foldmodifier 1 (Ufm1), by forming a high-energy thioester bond. It localizesmainly in cytoplasm, while it mainly localizes to the nucleus in presence ofSUMO2.
• Form: Liquid. In 20mMTris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 50mM NaCl.
• Molecular Weight: 47.4kDa(428aa) confirmed by MALDI-TOF.
• Purity: > 90%by SDS-PAGE
• Concentration: 0.5mg/ml (determined by Bradford assay)
• Sequences of aminoacids: MGSSHHHHHHSSGLVPRGSH MGSHMAESVE RLQQRVQELE RELAQERSLQ VPRSGDGGGG RVRIEKMSSEVVDSNPYSRL MALKRMGIVS DYEKIRTFAV AIVGVGGVGS VTAEMLTRCG IGKLLLFDYD KVELANMNRLFFQPHQAGLS KVQAAEHTLR NINPDVLFEV HNYNITTVEN FQHFMDRISN GGLEEGKPVD LVLSCVDNFEARMTINTACN ELGQTWMESG VSENAVSGHI QLIIPGESAC FACAPPLVVA ANIDEKTLKR EGVCAASLPTTMGVVAGILV QNVLKFLLNF GTVSFYLGYN AMQDFFPTMS MKPNPQCDDR NCRKQQEEYK KKVAALPKQEVIQEEEEIIH EDNEWGIELV SEVSEEELKN FSGPVPDLPE GITVAYTIPK KQEDSVTELT VEDSGESLEDLMAKMKNM
• Storage: Can bestored at +4°C short term (1-2 weeks). For long term storage, aliquot andstore at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: UBA5 ubiquitin-like modifieractivating enzyme 5 [ Homo sapiens ]
• Official Symbol: UBA5
• Synonyms: UBA5; ubiquitin-like modifier activatingenzyme 5; THIFP1; ThiFP1; UBE1DC1; FLJ23251; FLJ17281; FLJ23251UBA5;ubiquitin-like modifier-activating enzyme 5; UFM1-activating enzyme;ubiquitin-activating enzyme 5; UBA5, ubiquitin-activating enzyme E1 homolog;ubiquitin-activating enzyme E1-domain containing 1; ubiquitin-activatingenzyme E1 domain-containing protein 1; OTTHUMP00000216393;OTTHUMP00000216394; OTTHUMP00000217048; OTTHUMP00000217050
• Gene ID: 79876
• mRNA Refseq: NM_024818
• Protein Refseq: NP_079094
• MIM: 610552
• UniProt ID: Q9GZZ9
• Chromosome Location: 3q22.1
• Pathway: Adaptive Immune System; Antigen processing;Class I MHC mediated antigen processing & presentation; Immune System
• Function: ATP binding; UFM1 activating enzymeactivity; catalytic activity; cofactor binding; metal ion binding; nucleotidebinding; oxidoreductase activity, acting on the CH-OH group of donors, NAD orNADP as acceptor; protein binding