Recombinant Human Ubiquitin-Conjugating Enzyme E2D 4 (putative), His-tagged

• Cat.No.: UBE2D4-2494H
• Product Overview: Recombinant human UBE2D4 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques. MW: 18.8 kDa.

Specification

• Description: UBE2D4 belongs to the ubiquitin-conjugating enzyme family. Ubiquitin-conjugating enzymes, also known as E2 enzymes and more rarely as ubiquitin-carrier enzymes, perform the second step in the ubiquitination reaction that targets a protein for degradation via the proteasome. UBE2D4 accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 10% glycerol, 100mM NaCl.
• Molecular Mass: 18.8kDa (167aa), confirmed by MALDI-TOF.
• Protein length: 1-147aa
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MALKRIQKEL TDLQRDPPAQ CSAGPVGDDL FHWQATIMGP NDSPYQGGVF FLTIHFPTDY PFKPPKVAFTTKIYHPNINS NGSICLDILR SQWSPALTVS KVLLSICSLL CDPNPDDPLV PEIAHTYKAD REKYNRLARE WTQKYAM
• Purity: >95% by SDS - PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at 4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Concentration: 1mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: UBE2D4 ubiquitin-conjugating enzyme E2D 4 (putative) [ Homo sapiens ]
• Official Symbol: UBE2D4
• Synonyms: UBE2D4; ubiquitin-conjugating enzyme E2D 4 (putative); ubiquitin-conjugating enzyme E2 D4; HBUCE1; ubiquitin-protein ligase D4; ubiquitin carrier protein D4; ubiquitin-conjugating enzyme HBUCE1; FLJ32004
• Gene ID: 51619
• mRNA Refseq: NM_015983
• Protein Refseq: NP_057067
• UniProt ID: Q9Y2X8
• Chromosome Location: 7p13
• Pathway: Adaptive Immune System, organism-specific biosystem; Antigen processing: Ubiquitination & Proteasome degradation, organism-specific biosystem; Class I MHC mediated antigen processing & presentation, organism-specific biosystem; Immune System, organism-specific biosystem; Protein processing in endoplasmic reticulum, organism-specific biosystem
• Function: ATP binding; acid-amino acid ligase activity; ligase activity; nucleotide binding; protein binding; ubiquitin-protein ligase activity