Recombinant Human UBE2Z, His-tagged

• Cat.No.: UBE2Z-233H
• Product Overview: UBE2Z, 1-246aa, Human, His tag, E.coli

Specification

• Description: UBE2z belongs to the ubiquitin-conjugating enzyme family. This protein catalyzes the covalent attachment of ubiquitin to other proteins. It is a specific substrate for UBA6, not charged with ubiquitin by UBE1 and may be involved in apoptosis regulation. Recombinant human UBE2Z protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
• Source: E.coli
• Species: Human
• Tag: His-tag
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol, 1mM DTT
• Molecular Mass: 30.5 kDa (269aa), confirmed by MALDI-TOF
• Protein length: 1-246aa
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSMSIYKEP PPGMFVVPDT VDMTKIHALI TGPFDTPYEG GFFLFVFRCP PDYPIHPPRV KLMTTGNNTV RFNPNFYRNG KVCLSILGTW TGPAWSPAQS ISSVLISIQS LMTENPYHNE PGFEQERHPG DSKNYNECIR HETIRVAVCD MMEGKCPCPE PLRGVMEKSF LEYYDFYEVA CKDRLHLQGQ TMQDPFGEKR GHFDYQSLLM RLGLIRQKVL ERLHNENAEM DSDSSSSGTE TDLHGSLRV
• Purity: >95% by SDS - PAGE
• Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.25 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: UBE2Z ubiquitin-conjugating enzyme E2Z [ Homo sapiens ]
• Official Symbol: UBE2Z
• Synonyms: UBE2Z; ubiquitin-conjugating enzyme E2Z; ubiquitin-conjugating enzyme E2 Z; FLJ13855; UBA6 specific enzyme E2; USE1; UBA6-specific enzyme E2; ubiquitin-protein ligase Z; ubiquitin carrier protein Z; uba6-specific E2 conjugating enzyme 1; HOYS7
• Gene ID: 65264
• mRNA Refseq: NM_023079
• Protein Refseq: NP_075567
• MIM: 611362
• UniProt ID: Q9H832
• Chromosome Location: 17q21.32
• Pathway: Adaptive Immune System, organism-specific biosystem; Antigen processing: Ubiquitination and Proteasome degradation, organism-specific biosystem; Class I MHC mediated antigen processing & presentation, organism-specific biosystem; Immune System, organism-specific biosystem; Ubiquitin mediated proteolysis, organism-specific biosystem
• Function: ATP binding; acid-amino acid ligase activity; ligase activity; nucleotide binding; ubiquitin-protein ligase activity