Recombinant Human UBE2S Protein (N2-L222), His/Strep tagged

• Cat.No.: UBE2S-0012H
• Product Overview: Recombinant Human 8His-StrepII-TEV-(GG)-UBE2S(N2-L222 end) Protein was expressed in Insect cell.

Specification

• Description: Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Catalyzes 'Lys-11'-linked polyubiquitination. Acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated ubiquitin ligase that controls progression through mitosis. Acts by specifically elongating 'Lys-11'-linked polyubiquitin chains initiated by the E2 enzyme UBE2C/UBCH10 on APC/C substrates, enhancing the degradation of APC/C substrates by the proteasome and promoting mitotic exit. Also acts by elongating ubiquitin chains initiated by the E2 enzyme UBE2D1/UBCH5 in vitro; it is however unclear whether UBE2D1/UBCH5 acts as an E2 enzyme for the APC/C in vivo. Also involved in ubiquitination and subsequent degradation of VHL, resulting in an accumulation of HIF1A. In vitro able to promote polyubiquitination using all 7 ubiquitin Lys residues, except 'Lys-48'-linked polyubiquitination.
• Source: Insect cell
• Species: Human
• Tag: His&Strep
• Form: Liquid
• Protein length: N2-L222
• Endotoxin: < 0.01 EU per μg of the protein
• Purity: 90%
• Stability: Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade.
• Storage: Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Supplied as sterile 50 mM Tris-HCl (pH7.5), 200 mM NaCl, 20% glycerol
• Shipping: It is shipped out with blue ice.

Gene Information

• Gene Name: UBE2S ubiquitin-conjugating enzyme E2S [ Homo sapiens (human) ]
• Official Symbol: UBE2S
• Synonyms: UBE2S; ubiquitin-conjugating enzyme E2S; ubiquitin-conjugating enzyme E2 S; E2 EPF; ubiquitin carrier protein; ubiquitin conjugating enzyme E2 24 kD; ubiquitin protein ligase; E2-EPF5; ubiquitin-protein ligase S; ubiquitin carrier protein S; ubiquitin-conjugating enzyme E2-EPF5; ubiquitin-conjugating enzyme E2-24 kD; ubiquitin-conjugating enzyme E2-24 kDa; EPF5; E2EPF; E2-EPF
• Gene ID: 27338
• mRNA Refseq: NM_014501
• Protein Refseq: NP_055316
• MIM: 610309
• UniProt ID: Q16763