Active Recombinant Human UBE2S Protein, His-tagged

• Cat.No.: UBE2S-684H
• Product Overview: Recombinant Human UBE2S (Accession # NP_055316.2), fused with an N-terminal 6-His tag, was produced in E. coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Form: X mg/ml (x µM) in 50 mM HEPES pH 7.5, 200 mM NaCl, 10% Glycerol (v/v), 1 mM TCEP
• Bio-activity: Recombinant Human His6-UBE2S is a member of the Ubiquitin-conjugating (E2) enzyme family that receives Ubiquitin from a Ubiquitin-activating (E1) enzyme and subsequently interacts with a Ubiquitin ligase (E3) to conjugate Ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human His6-UBE2S concentration of 0.1-1 µM.
• Molecular Mass: Recombinant Human UBE2S, His-tagged has a calculated MW of 36 kDa.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: UBE2S ubiquitin-conjugating enzyme E2S [ Homo sapiens ]
• Official Symbol: UBE2S
• Synonyms: UBE2S; ubiquitin-conjugating enzyme E2S; ubiquitin-conjugating enzyme E2 S; E2 EPF; ubiquitin carrier protein; ubiquitin conjugating enzyme E2 24 kD; ubiquitin protein ligase; E2-EPF5; ubiquitin-protein ligase S; ubiquitin carrier protein S; ubiquitin-conjugating enzyme E2-EPF5; ubiquitin-conjugating enzyme E2-24 kD; ubiquitin-conjugating enzyme E2-24 kDa; EPF5; E2EPF; E2-EPF
• Gene ID: 27338
• mRNA Refseq: NM_014501
• Protein Refseq: NP_055316
• MIM: 610309
• UniProt ID: Q16763
• Chromosome Location: 19q13.43