Recombinant Human UBE2K Protein (A2-N200), His/Strep tagged

• Cat.No.: UBE2K-0045H
• Product Overview: Recombinant Human 8His-StrepII-TEV-(GG)-UBE2K(A2-N200 end) Protein was expressed in Insect cell.

Specification

• Description: Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro, in the presence or in the absence of BRCA1-BARD1 E3 ubiquitin-protein ligase complex, catalyzes the synthesis of 'Lys-48'-linked polyubiquitin chains. Does not transfer ubiquitin directly to but elongates monoubiquitinated substrate protein. Mediates the selective degradation of short-lived and abnormal proteins, such as the endoplasmic reticulum-associated degradation (ERAD) of misfolded lumenal proteins. Ubiquitinates huntingtin. May mediate foam cell formation by the suppression of apoptosis of lipid-bearing macrophages through ubiquitination and subsequence degradation of p53/TP53. Proposed to be involved in ubiquitination and proteolytic processing of NF-kappa-B; in vitro supports ubiquitination of NFKB1. In case of infection by cytomegaloviruses may be involved in the US11-dependent degradation of MHC class I heavy chains following their export from the ER to the cytosol. In case of viral infections may be involved in the HPV E7 protein-dependent degradation of RB1.
• Source: Insect cell
• Species: Human
• Tag: His&Strep
• Form: Liquid
• Protein length: A2-N200
• Endotoxin: < 0.01 EU per μg of the protein
• Purity: 90%
• Stability: Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade.
• Storage: Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Supplied as sterile 50 mM Tris-HCl (pH7.5), 200 mM NaCl, 20% glycerol
• Shipping: It is shipped out with blue ice.

Gene Information

• Gene Name: UBE2K ubiquitin-conjugating enzyme E2K [ Homo sapiens (human) ]
• Official Symbol: UBE2K
• Synonyms: UBE2K; ubiquitin-conjugating enzyme E2K; HIP2, huntingtin interacting protein 2, ubiquitin conjugating enzyme E2K (UBC1 homolog, yeast); ubiquitin-conjugating enzyme E2 K; HYPG; UBC1; HIP-2; E2(25K); ubiquitin-protein ligase; ubiquitin carrier protein; huntingtin interacting protein 2; huntingtin-interacting protein 2; ubiquitin-conjugating enzyme E2-25K; ubiquitin-conjugating enzyme E2(25K); ubiquitin-conjugating enzyme E2-25 KDA; ubiquitin-conjugating enzyme E2K (UBC1 homolog, yeast); LIG; HIP2; E2-25K; DKFZp564C1216; DKFZp686J24237
• Gene ID: 3093
• mRNA Refseq: NM_001111112
• Protein Refseq: NP_001104582
• MIM: 602846
• UniProt ID: P61086