Recombinant Human UBE2F, His-tagged

• Cat.No.: UBE2F-207H
• Product Overview: Recombinant full length human UBE2F was expressed in E. coli cells using an N-terminal His tag.

Specification

• Description: UBE2F or ubiquitin-conjugating enzyme E2F is part of the ubiquitin network for protein degradation and is involved in protein neddylation. UBE2F has been shown to function as a NEDD8-conjugating enzyme in vitro and in vivo. Covalent attachment of the ubiquitin-like protein NEDD8 to a conserved C-terminal domain (ctd) lysine stimulates Cullin Ring ligase (CRL) ubiquitination activity and prevents binding of the inhibitor CAND1.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Recombinant protein stored in 50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, 25% glycerol.
• Molecular Mass: ~24 kDa
• Purity: >95% by densitometry
• Applications: Western Blot
• Storage: Store product at –70 centigrade. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Concentration: 0.1 μg/μl

Gene Information

• Gene Name: UBE2F ubiquitin-conjugating enzyme E2F (UBC6 homolog, C. elegans) [ Homo sapiens ]
• Official Symbol: UBE2F
• Synonyms: UBE2F; ubiquitin-conjugating enzyme E2F (UBC6 homolog, C. elegans); NCE2; NEDD8 conjugating enzyme
• Gene ID: 23665
• UniProt ID: Q969M7
• Chromosome Location: 2q37.3
• Pathway: Cell cycle, organism-specific biosystem