Recombinant Human Twinfilin, Actin-Binding Protein, Homolog 1 (Drosophila), GST-tagged

• Cat.No.: TWF1-4767H
• Product Overview: A DNA sequence encoding the human TWF1 isoform 4 ( Q12792-4 ) ( Met 1 - Asp 252 ) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Specification

• Species: Human
• Source: Human
• Tag: GST
• ProteinLength: 1-252 a.a.
• Description: TWF1 is a cytoplasm protein which belongs to the actin-binding proteins ADF family and Twinfilin subfamily. In addition to the mammalian twinfilin-1, a second protein with approximately 65% sequence identity to twinfilin-1 exists in mouse and humans. TWF1 is expressed at high levels in the colon, testis, ovary, prostate and lung.
• Form: Lyophilized from sterile 50mM Tris, 500mM NaCl, pH 8.0. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
• Purity: > 84 % as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70°C.
• Predicted N terminal: Met
• Molecular Mass: The recombinant human TWF1/GST chimera consists of 494 amino acids and has a calculated molecular mass of 57 kDa. It migrates as an approximately 50 kDa band in SDS-PAGE under reducing conditions.
• Storage: Store it under sterile conditions at -70°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Pathways: superpathway of gluconate degradation

Gene Information

• Gene Name: TWF1 twinfilin, actin-binding protein, homolog 1 (Drosophila) [ Homo sapiens ]
• Official Symbol: TWF1
• Synonyms: TWF1; twinfilin, actin-binding protein, homolog 1 (Drosophila); A6; PTK9; MGC23788; MGC41876; twinfilin-1; protein A6; protein tyrosine kinase 9; A6 protein tyrosine kinase; PTK9 protein tyrosine kinase 9; Twinfilin-1; Protein A6; Protein tyrosine kinase 9
• Gene ID: 5756
• mRNA Refseq: NM_001242397
• Protein Refseq: NP_001229326
• MIM: 610932
• UniProt ID: Q12792
• Chromosome Location: 12q12
• Function: ATP binding; actin monomer binding; phosphatidylinositol-4,5-bisphosphate binding; protein tyrosine kinase activity; NOT protein tyrosine kinase activity