Recombinant Human Tumor Protein P53, GST-tagged

• Cat.No.: TP53-325H
• Product Overview: Recombinant full-length human p53 was expressed by baculovirus in Hi5 cells using an N-terminal GST tag.

Specification

• Species: Human
• Source: Sf9 Cells
• Tag: GST
• Description: TP53 is a key regulator of cell growth and acts as a tumor suppressor gene. Wild-type TP53 gene can suppress transformation of rat embryo fibroblasts in cell culture by other oncogenes such as the adenovirus E1A and Ras. DNA tumor viruses such as SV40 large T antigen and the adenovirus E1A plus E1B-55Kd proteins bind to TP53 and inactivate its tumor suppressor activities leading to cellular transformation. Mutational inactivation of the TP53 gene is detected in more than 50% of human cancers. Mutation of TP53 renders cancer cells more resistant to current cancer therapies due to lack of TP53-mediated apoptosis.
• Applications: Kinase Assay; Western Blot
• Molecular Weight: 79 kDa
• Expression System: Sf9 insect cells using baculovirus
• Form: Recombinant protein stored in 50mM sodium phosphate, pH 7.0, 300mM NaCl, 150 mM imidazole, 0.1 mM PMSF, 0.25 mM DTT, 25% glycerol.
• Purity: > 95 %
• Concentration: 0.2 ug/ul
• Sequences: Full-length
• Storage: Store product at -70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Gene Information

• Gene Name: TP53 tumor protein p53 [ Homo sapiens ]
• Official Symbol: TP53
• Synonyms: TP53; tumor protein p53; p53; LFS1; TRP53; FLJ92943; Antigen NY-CO-13; Phosphoprotein p53; Tumor suppressor p53; p53 transformation suppressor; p53 tumor suppressor; transformation-related protein 53
• Gene ID: 7157
• mRNA Refseq: NM_00546
• Protein Refseq: NP_000537
• MIM: 191170
• UniProt ID: P04637
• Chromosome Location: 17p13.1
• Function: ATP binding; DNA strand annealing activity; chaperone binding; chromatin binding; copper ion binding; enzyme binding; metal ion binding; promoter binding; protein N-terminus binding; protein heterodimerization activity; transcription factor activity; transcription factor binding; zinc ion binding