Recombinant Human Tumor Necrosis Factor, HQ-tagged

• Cat.No.: TNF-1914H
• Product Overview: Recombinant humanTNF-alpha protein contains 158 amino acid residues and a 16 aa HQ tag. Totallength is 174 aa.

Specification

• Species: Human
• Source: Hordeum Vulgare
• Tag: HQ
• Description: Tumor necrosis factor-alphais the best-known member of this class, and sometimes referred to when theterm tumor necrosis factor is used. TNF is a monocyte-derived cytotoxin thathas been implicated in tumour regression, septic shock and cachexia.
• Form: Lyophilized from a0.2 μm sterile filtered solution of PBS, pH 7.2.
• Purity: >98% by SDS-PAGE.Purified product carries no pyrogenic or pro-inflammatory contaminants, asassayed with monocyte activation test using custom human Multiplex CytokineAssay measuring IL-6, TNF-alpha and IL-1beta induction.
• Molecular Weight: Projected MW 19.6kDa. The recombinant protein migrates as two bands with an apparent MW 21 and23 kDa in SDS-PAGE.
• Endotoxin Level: < 0.005 ng per μgof product (0.05 EU/μg) as measured by turbidimetric kinetic assay.
• Reconstitution: Centrifuge vialprior to opening. First add sterile distilled water to the vial to fullysolubilize the protein to a concentration not less than 100 μg/ml. Aftercomplete solubilization of the protein, it can be further diluted to otheraqueous solutions.
• Biological Activity: The ED50is determined by the Cytotoxicity Assay for the human-TNF-alpha fordosedependent cytolytic effects of serial dilutions of h-TNF-alpha on thefibrosarcoma L-929 cells. The ED50 for a typical batch ofrecombinant human TNF-alpha is 40 ng/ml.
• Storage: The lyophilizedprotein, though stable at room temperature for two weeks, is best stored at-20°C. Reconstituted protein should be used immediately or stored in workingaliquots at -20°C. Avoid repeated freeze-thaw cycles.
• OfficialSymbol: TNF
• Pathways: Adipocytokinesignaling pathway; Adipogenesis; African trypanosomiasis; Allograftrejection; Alzheimer" s disease; Amoebiasis; Amyotrophic lateral sclerosis(ALS); Angiopoietin receptor Tie2-mediated signaling; Antigen processing andpresentation; Apoptosis; Asthma; Calcineurin-regulated NFAT-dependenttranscription in lymphocytes; Canonical NF-kappaB pathway; Caspase cascade inapoptosis; Cellular roles of Anthrax toxin; Ceramide signaling pathway

Gene Information

• Gene Name: TNF tumor necrosis factor [ Homosapiens ]
• Synonyms: TNF;tumor necrosis factor; DIF; TNFA; TNFSF2; TNF-alpha; TNF-a; cachectin;APC1 protein; OTTHUMP00000029281; TNF, monocyte-derived; TNF,macrophage-derived; TNF superfamily, member 2; tumor necrosis factor alpha;tumor necrosis factor-alpha; tumor necrosis factor ligand superfamily member2; Tumornecrosis factor; Cachectin
• Gene ID: 7124
• mRNA Refseq: NM_000594
• Protein Refseq: NP_000585
• MIM: 191160
• UniProt ID: P01375
• Chromosome Location: 6p21.3
• Function: cytokine activity; identicalprotein binding; protease binding; protein binding; transcription regulatoryregion DNA binding; tumor necrosis factor receptor binding