Recombinant Human TUBB3 protein, His & GST-tagged

• Cat.No.: TUBB3-1305H
• Product Overview: Recombinant Human TUBB3 aa. (Tyr222~Glu438 fused with N-terminal His & GST tag was produced in E. coli cells.

Specification

• Description: This gene encodes a class III member of the beta tubulin protein family. Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. This protein is primarily expressed in neurons and may be involved in neurogenesis and axon guidance and maintenance. Mutations in this gene are the cause of congenital fibrosis of the extraocular muscles type 3. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 6.
• Source: E. coli
• Species: Human
• Tag: His&GST
• Form: Freeze-dried powder
• Molecular Mass: 54kDa as determined by SDS-PAGE reducing conditions.
• Protein length: Tyr222~Glu438
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: >92%
• Characteristic: The isoelectric point is 6.1.
• Applications: SDS-PAGE; WB; ELISA; IP; CoIP; Purification; Amine Reactive Labeling.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Concentration: 200μg/mL
• Storage buffer: PBS, pH7.4, containing 1mM DTT, 5% trehalose, 0.01% sarcosyl and Proclin300.
• Reconstitution: Reconstitute in PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: TUBB3 tubulin beta 3 class III [ Homo sapiens (human) ]
• Official Symbol: TUBB3
• Synonyms: TUBB3; tubulin beta 3 class III; CDCBM; FEOM3; TUBB4; CDCBM1; CFEOM3; beta-4; CFEOM3A; tubulin beta-3 chain; class III beta-tubulin; tubulin beta-4 chain; tubulin beta-III; tubulin, beta 3
• Gene ID: 10381
• mRNA Refseq: NM_001197181.1
• Protein Refseq: NP_001184110.1
• UniProt ID: Q13509