Recombinant Human TUBB3, His-tagged

• Cat.No.: TUBB3-7552H
• Product Overview: Recombinant human TUBB3 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Specification

• Antigen Description: Tubulin, beta 3 class III, also known as TUBB3, is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. TUBB3 plays a critical role in proper axon guidance and mantainance.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl,10% glycerol, 1mM DTT
• Molecular Mass: 52.8 kDa (473aa)
• Protein length: 1-450aa
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSMREIVHI QAGQCGNQIG AKFWEVISDE HGIDPSGNYV GDSDLQLERI SVYYNEASSH KYVPRAILVD LEPGTMDSVR SGAFGHLFRP DNFIFGQSGA GNNWAKGHYT EGAELVDSVL DVVRKECENC DCLQGFQLTH SLGGGTGSGM GTLLISKVRE EYPDRIMNTF SVVPSPKVSD TVVEPYNATL SIHQLVENTD ETYCIDNEAL YDICFRTLKL ATPTYGDLNH LVSATMSGVT TSLRFPGQLN ADLRKLAVNM VPFPRLHFFM PGFAPLTARG SQQYRALTVP ELTQQMFDAK NMMAACDPRH GRYLTVATVF RGRMSMKEVD EQMLAIQSKN SSYFVEWIPN NVKVAVCDIP PRGLKMSSTF IGNSTAIQEL FKRISEQFTA MFRRKAFLHW YTGEGMDEME FTEAESNMND LVSEYQQYQD ATAEEEGEMY EDDEEESEAQ GPK
• Purity: >90% as determined by SDS - PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at 4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C.Avoid repeated freezing and thawing cycles.
• Concentration: 0.25 mg/ml

Gene Information

• Gene Name: TUBB3 tubulin, beta 3 class III [ Homo sapiens ]
• Official Symbol: TUBB3
• Synonyms: TUBB3; tubulin, beta 3 class III; tubulin, beta 3; tubulin beta-3 chain; beta 4; class III beta tubulin; tubulin beta-III; tubulin beta-4 chain; class III beta-tubulin; CDCBM; TUBB4; beta-4; CFEOM3A
• Gene ID: 10381
• mRNA Refseq: NM_001197181
• Protein Refseq: NP_001184110
• MIM: 602661
• UniProt ID: Q13509
• Chromosome Location: 16q24.3
• Pathway: Chaperonin-mediated protein folding, organism-specific biosystem; Class A/1 (Rhodopsin-like receptors), organism-specific biosystem; Cooperation of Prefoldin and TriC/CCTin actin and tubulin folding, organism-specific biosystem; Diurnally regulated genes with circadian orthologs, organism-specific biosystem; Formation of tubulin folding intermediates by CCT/TriC, organism-specific biosystem; G alpha (s) signalling events, organism-specific biosystem; GPCR downstream signaling, organism-specific biosystem
• Function: GTP binding; GTPase activity; nucleotide binding; structural molecule activity