Recombinant Human TTL Protein, His&GST-tagged

• Cat.No.: TTL-506H
• Product Overview: Recombinant Human TTL Protein (Met1-Leu377) with N-His&GST tag was expressed in E. coli.

Specification

• Description: TTL is a cytosolic enzyme involved in the posttranslational modification of alpha-tubulin (see MIM 602529). Alpha-tubulin within assembled microtubules is detyrosinated over time at the C terminus. After microtubule disassembly, TTL restores the tyrosine residues and consequently participates in a cycle of tubulin detyrosination and tyrosination.
• Source: E. coli
• Species: Human
• Tag: His&GST
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 73.2 kDa
  Accurate Molecular Mass: 73 kDa
• Protein length: Met1-Leu377
• Purity: > 80%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: PBS, pH7.4, containing 0.01% SKL, 1 mM DTT, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in sterile water to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: TTL tubulin tyrosine ligase [ Homo sapiens (human) ]
• Official Symbol: TTL
• Synonyms: TTL; tubulin tyrosine ligase; tubulin--tyrosine ligase; MGC46235; 2410003M22Rik; tubulin-tyrosine ligase
• Gene ID: 150465
• mRNA Refseq: NM_153712
• Protein Refseq: NP_714923
• MIM: 608291
• UniProt ID: Q8NG68