Recombinant Human TSTA3, His-tagged

• Cat.No.: TSTA3-2763H
• Product Overview: Recombinant human TSTA3 protein, fused to His-tag at N-terminus, was expressed inE.coliand purified by using conventional chromatography techniques.

Specification

• Cat. No.: TSTA3-2763H
• Description: TSTA3 is a NADP(H)-binding protein. It catalyzes the two-step epimerase and the reductase reactions in GDP-D-mannose metabolism, converting GDP-4-keto-6-D-deoxymannose to GDP-L-fucose. GDP-L-fucose is the substrate of several fucosyltransferases involved in the expression of many glycoconjugates, including blood group ABH antigens and developmental adhesion antigens. Mutations in this gene may cause leukocyte adhesion deficiency, type II.
• Source: E.coli.
• Purity: > 90% by SDS – PAGE.
• Sequences: MGSSHHHHHH SSGLVPRGSH MGEPQGSMRI LVTGGSGLVG KAIQKVVADG AGLPGEDWVF VSSKDADLTD TAQTRALFEK VQPTHVIHLA AMVGGLFRNI KYNLDFWRKN VHMNDNVLHS AFEVGARKVV SCLSTCIFPD KTTYPIDETM IHNGPPHNSN FGYSYAKRMI DVQNRAYFQQ YGCTFTAVIP TNVFGPHDNF NIEDGHVLPG LIHKVHLAKS SGSALTVWGT GNPRRQFIYS LDLAQLFIWV LREYNEVEPI ILSVGEEDEV SIKEAAEAVV EAMDFHGEVT FDTTKSDGQF KKTASNSKLR TYLPDFRFTP FKQAVKETCA WFTDNYEQAR K.
• Molecular Weight: 38.0 kDa (341aa)confirmed by MALDI-TOF.
• Form: Liquid. 20mM Tris-HCl buffer (pH8.0) containing 10% glycerol, 2mM DTT, 50mM NaCl.
• Concentration: 0.5 mg/ml (determined by Bradford assay).
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: TSTA3 tissue specific transplantation antigen P35B [ Homo sapiens ]
• Synonyms:
  TSTA3; tissue specific transplantation antigen P35B; 3-5 epimerase/4-reductase; GDP-4-keto-6-deoxy-D-mannose epimerase-reductase; GDP-4-keto-6-deoxy-D-mannose-3,5-epimerase-4-reductase; GDP-L-fucose synthase; Tissue-specific transplantation antigen-3; protein FX; red cell NADP(H)-binding protein; short chain dehydrogenase/reductase family 4E, member 1; short-chain dehydrogenase/reductase family 4E member 1; tissue specific transplantation antigen 3; FX; P35B; SDR4E1; EC 1.1.1.271
• Gene ID: 7264
• mRNA Refseq: NM_003313
• Protein Refseq: NP_003304
• MIM: 137020
• UniProt ID: Q13630
• Chromosome Location: 8q24.3
• Pathway: Amino sugar and nucleotide sugar metabolism;Fructose and mannose metabolism;Metabolic pathways
• Function: GDP-4-dehydro-D-rhamnose reductase activity; GDP-L-fucose synthase activity; catalytic activity; electron carrier activity; oxidoreductase activity