Recombinant Human Tryptophan 2,3-Dioxygenase, His-tagged

• Cat.No.: TDO2-2503H
• Product Overview: Recombinant human TDO2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques. MW: 50.0 kDa.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 1-406aa
• Description: TDO2, also known as TDO, is a ferrous heme enzyme that catalyzes the first and rate-limiting step in the kynurenine pathway, the major pathway of tryptophan metabolism. It incorporates oxygen into the indole moiety of tryptophan. It has a broad specificity towards tryptamine and derivatives including D- and L-tryptophan, 5-hydroxytryptophan and serotonin.
• Form: Liquid. In 20 mM Tris-HCl buffer (pH8.0) containing 0.2M NaCl, 5mM DTT, 1mM EDTA, 30% glycerol.
• Molecular Mass: 50.0 kDa (426aa), confirmed by MALDI-TOF
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MSGCPFLGNN FGYTFKKLPV EGSEEDKSQT GVNRASKGGL IYGNYLHLEK VLNAQELQSE TKGNKIHDEH LFIITHQAYE LWFKQILWEL DSVREIFQNG HVRDERNMLK VVSRMHRVSV ILKLLVQQFS ILETMTALDF NDFREYLSPA SGFQSLQFRL LENKIGVLQN MRVPYNRRHY RDNFKGEENE LLLKSEQEKT LLELVEAWLE RTPGLEPHGF NFWGKLEKNI TRGLEEEFIR IQAKEESEEK EEQVAEFQKQ KEVLLSLFDE KRHEHLLSKG ERRLSYRALQ GALMIYFYRE EPRFQVPFQL LTSLMDIDSL MTKWRYNHVC MVHRMLGSKA GTGGSSGYHY LRSTVSDRYK VFVDLFNLST YLIPRHWIPK MNPTIHKFLY TAEYCDSSYF SSDESD
• Purity: >90% by SDS-PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at 4℃ short term (1-2 weeks). For long term storage, aliquot and store at -20℃ or -70℃. Avoid repeated freezing and thawing cycles.
• Concentration: 0.25 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: TDO2 tryptophan 2,3-dioxygenase [ Homo sapiens ]
• Official Symbol: TDO2
• Synonyms: TDO2; tryptophan 2,3-dioxygenase; TDO; TPH2; tryptophanase; tryptophan oxygenase; tryptophan pyrrolase; tryptamin 2,3-dioxygenase; TO; TRPO
• Gene ID: 6999
• mRNA Refseq: NM_005651
• Protein Refseq: NP_005642
• MIM: 191070
• UniProt ID: P48775
• Chromosome Location: 4q31-q32
• Pathway: Metabolic pathways, organism-specific biosystem; Metabolism, organism-specific biosystem; Metabolism of amino acids and derivatives, organism-specific biosystem; Monoamine Transport, organism-specific biosystem; NAD biosynthesis II (from tryptophan), organism-specific biosystem; NAD biosynthesis II (from tryptophan), conserved biosystem; Tryptophan catabolism, organism-specific biosystem
• Function: amino acid binding; heme binding; metal ion binding; oxidoreductase activity; oxygen binding; tryptophan 2,3-dioxygenase activity