Recombinant Human troponin I type 2 (skeletal, fast), His-tagged

• Cat.No.: TNNI2-371H
• Product Overview: TNNI2, 1-182aa, Human, His tag, E.coli

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 1-182 a.a.
• Description: Troponin I, fast skeletal muscle, also known as TNNI2, is a member of the troponin I gene family, and a component of the troponin complex including troponin T, troponin C and troponin I subunits. The troponin complex, along with tropomyosin, is responsible for the calcium-dependent regulation of striated muscle contraction. This protein also suppresses tumor growth in human ovarian carcinoma. Mutations in TNNI2 cause myopathy and distal arthrogryposis type 2B. Recombinant human TNNI2 protein, fused to His-tag at N-terminus, was expressed in E.coli.
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 2M Urea, 20% glycerol, 0.2M NaCl
• Molecular Mass: 23.5 kDa (202aa)
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGDEEKRNRA ITARRQHLKS VMLQIAATEL EKEESRREAE KQNYLAEHCP PLHIPGSMSE VQELCKQLHA KIDAAEEEKY DMEVRVQKTS KELEDMNQKL FDLRGKFKRP PLRRVRMSAD AMLKALLGSK HKVCMDLRAN LKQVKKEDTE KERDLRDVGD WRKNIEEKSG MEGRKKMFES ES
• Purity: >90% by SDS - PAGE
• Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.25 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: TNNI2 troponin I type 2 (skeletal, fast) [ Homo sapiens ]
• Official Symbol: TNNI2
• Synonyms: TNNI2; troponin I type 2 (skeletal, fast); troponin I, skeletal, fast; troponin I, fast skeletal muscle; AMCD2B; DA2B; FSSV; troponin I fast twitch 2; troponin I; fast twitch skeletal muscle isoform; troponin I, fast-twitch isoform; fast-twitch skeletal muscle troponin I; troponin I, fast-twitch skeletal muscle isoform; fsTnI
• Gene ID: 7136
• mRNA Refseq: NM_001145829
• Protein Refseq: NP_001139301
• MIM: 191043
• UniProt ID: P48788
• Chromosome Location: 11p15.5
• Pathway: Muscle contraction, organism-specific biosystem; Striated Muscle Contraction, organism-specific biosystem; Striated Muscle Contraction, organism-specific biosystem
• Function: contributes_to actin binding; protein binding; troponin T binding