Recombinant Human TRMT12 Protein, His-tagged

• Cat.No.: TRMT12-2806H
• Product Overview: Recombinant Human TRMT12 protein, fused to His-tagged at N-terminus, was expressed in E. coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: Wybutosine (yW) is a hypermodified guanosine at the 3-prime position adjacent to the anticodon of phenylalanine tRNA that stabilizes codon-anticodon interactions during decoding on the ribosome. TRMT12 is the human homolog of a yeast gene essential for yW synthesis (Noma and Suzuki, 2006).
• Form: 25 mM Tris, pH 8.0, 150 mM NaCl, 10% glycerol, 1% Sarkosyl. Store at -80 Centigrade. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions.
• Molecular Mass: 50.2 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: >50 ug/mL as determined by microplate BCA method

Gene Information

• Gene Name: TRMT12 tRNA methyltransferase 12 homolog (S. cerevisiae) [ Homo sapiens ]
• Official Symbol: TRMT12
• Synonyms: TRM12; TYW2
• Gene ID: 55039
• mRNA Refseq: NM_017956
• Protein Refseq: NP_060426
• MIM: 611244
• UniProt ID: Q53H54