Recombinant Human Triosephosphate Isomerase 1, His-tagged

• Cat.No.: TPI1-2845H
• Product Overview: Recombinant human TPI1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. MW =28.8kDa (269aa) confirmed by MALDI-TOF.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: TPI1 (Triosephosphate isomerase) belongs to the triosephosphate isomerase family. TPI1 catalyzes the isomerization of glyceraldehydes 3-phosphate (G3P) and dihydroxy-acetone phosphate (DHAP) in glycolysis and gluconeogenesis. Defects in TPI1 are the cause of triosephosphate isomerase deficiency (TPI deficiency). TPI deficiency is an autosomal recessive disorder. It is the most severe clinical disorder of glycolysis. It is associated with neonatal jaundice, chronic hemolytic anemia, progressive neuromuscular dysfunction, cardiomyopathy and increased susceptibility to infection.
• Sequences Of Amino Acids: MGSSHHHHHH SSGLVPRGSH MAPSRKFFVG GNWKMNGRKQ SLGELIGTLN AAKVPADTEV VCAPPTAYID FARQKLDPKI AVAAQNCYKV TNGAFTGEIS PGMIKDCGAT WVVLGHSERR HVFGESDELI GQKVAHALAE GLGVIACIGE KLDEREAGIT EKVVFEQTKV IADNVKDWSK VVLAYEPVWA IGTGKTATPQ QAQEVHEKLR GWLKSNVSDA VAQSTRIIYG GSVTGATCKE LASQPDVDGF LVGGASLKPE FVDIINAKQ
• Purity: > 95% by SDS – PAGE.
• Form: Liquid. 20mM Tris-HCl buffer (pH8.0) containing 10% glycerol, 1mM DTT.
• Concentration: 0.5mg/ml (determined by Bradford assay).
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: TPI1 triosephosphate isomerase 1 [ Homo sapiens ]
• Synonyms: TPI1; triosephosphate isomerase 1; TPI; MGC88108; triosephosphate isomerase; TIMOTTHUMP00000165244; triose-phosphate isomerase; EC 5.3.1.1
• Gene ID: 7167
• mRNA Refseq: NM_000365
• Protein Refseq: NP_000356
• MIM: 190450
• UniProt ID: P60174
• Chromosome Location: 12p13
• Pathway: Fructose and mannose metabolism; Glycolysis / Gluconeogenesis; Inositol phosphate metabolism; Metabolic pathways
• Function: isomerase activity; protein binding; triose-phosphate isomerase activity