Recombinant Human TRIM28, His-tagged

• Cat.No.: TRIM28-2325H
• Product Overview: Recombinant human TRIM28 protein, fused to His-tag at N-terminus, was expressed in E.coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 366-802aa
• Description: The protein encoded by this gene mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors. The protein localizes to the nucleus and is thought to associate with specific chromatin regions. The protein is a member of the tripartite motif family. This tripartite motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region.
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 0.4M Urea.
• Molecular Mass: 48.7 kDa (460aa)
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSKLIYFQL HRALKMIVDP VEPHGEMKFQ WDLNAWTKSA EAFGKIVAER PGTNSTGPAP MAPPRAPGPLSKQGSGSSQP MEVQEGYGFG SGDDPYSSAE PHVSGVKRSR SGEGEVSGLM RKVPRVSLER LDLDLTADSQ PPVFKVFPGS TTEDYNLIVIERGAAAAATG QPGTAPAGTP GAPPLAGMAI VKEEETEAAI GAPPTATEGP ETKPVLMALA EGPGAEGPRL ASPSGSTSSG LEVVAPEGTSAPGGGPGTLD DSATICRVCQ KPGDLVMCNQ CEFCFHLDCH LPALQDVPGE EWSCSLCHVL PDLKEEDGSL SLDGADSTGV VAKLSPANQRKCERVLLALF CHEPCRPLHQ LATDSTFSLD QPGGTLDLTL IRARLQEKLS PPYSSPQEFA QDVGRMFKQF NKLTEDKADV QSIIGLQRFFETRMNEAFGD
• Purity: > 90% by SDS - PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Concentration: 1 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: TRIM28 tripartite motif containing 28 [ Homo sapiens (human) ]
• Official Symbol: TRIM28
• Synonyms: TRIM28; KAP1; TF1B; RNF96; TIF1B; tripartite motif containing 28; transcription intermediary factor 1-beta; KAP-1; KRIP-1; TIF1-beta; RING finger protein 96; KRAB-associated protein 1; nuclear corepressor KAP-1; KRAB-interacting protein 1; E3 SUMO-protein ligase TRIM28; tripartite motif-containing 28; tripartite motif-containing protein 28; transcriptional intermediary factor 1-beta
• Gene ID: 10155
• mRNA Refseq: NM_005762
• Protein Refseq: NP_005753
• MIM: 601742
• UniProt ID: Q13263
• Chromosome Location: 19q13.4
• Pathway: C-MYB transcription factor network; E2F transcription factor network; Generic Transcription Pathway
• Function: DNA binding; Krueppel-associated box domain binding; chromo shadow domain binding