Recombinant Human Transforming Growth Factor, Beta 1, His-tagged

• Cat.No.: TGFB1-908H
• Product Overview: Recombinant Humantransforming growth factor, beta 1 encoding the human LAP/Prepro-transforming growth factor-beta 1 (Leu³⁰-Arg²⁷⁸) was recombined with his tag at the N-Terminus. That was expressed inE.Coli, and purified by Ni-NTA column. MW=27.4.7KD.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 30-278 a.a.
• Description: Transforming growth factor beta 1 or TGF-β1 is a polypeptide member of the transforming growth factor beta superfamily of cytokines. It is a secreted protein that performs many cellular functions, including the control of cell growth, cell proliferation, cell differentiation and apoptosis.
• Sequence: Human LAP/Prepro-transforming growth factor-beta 1 (Leu³⁰-Arg²⁷⁸).LSTCKTIDME LVKRKRIEAI RGQILSKLRL ASPPSQGEVP PGPLPEAVLA LYNSTRDRVA GESAEPEPEP EADYYAKEVT RVLMVETHNE IYDKFKQSTH SIYMFFNTSE LREAVPEPVL LSRAELRLLR LKLKVEQHVE LYQKYSNNSW RYLSNRLLAP SDSPEWLSFD VTGVVRQWLS RGGEIEGFRL SAHCSCDSRD NTLQVDINGF TTGRRGDLAT IHGMNRPFLL LMATPLERAQ HLQSSRHRR.
• Purity: >95% in SDS gel.
• Formulation: Lyophilized 100 μg human LAP/Prepro-transforming growth factor-beta 1in 50 μl of TBS ( 20 mM Tris, 50mM NaCl, pH8.0 ). Carry free.
• Reconstitution: Add 500μl deionized water to the vial to prepare a working stock solution at 200 μg/mL. Allow to set at least 30 minutes at 4℃, mix well.
• Application: Cell biology, ELISA.
• Storage: Store lyophilized protein at -20℃ to -70℃. Lyophilized protein is stable for up to 6 months from date of receipt at - 20℃ to -70℃. Upon reconstitution, this protein can be stored at -20℃ for up to a few weeks or at -70℃ in a manual defrost freezer for long term storage (six months). Aliquot reconstituted protein to avoid repeated freeze / thaw cycles.

Gene Information

• Gene Name: TGFB1 transforming growth factor, beta 1 [ Homo sapiens ]
• Synonyms: TGFB1; transforming growth factor, beta 1; CED; LAP; DPD1; TGFB; TGFbeta; TGF-beta 1 protein; latency-associated peptide; Camurati-Engelmann disease; Transforming growth factor beta-1; TGF-beta-1; Latency-associated peptide; LAP
• Gene ID: 7040
• mRNA Refseq: NM_000660
• Protein Refseq: NP_000651
• MIM: 190180
• UniProt ID: P01137
• Chromosome Location: 19q13.1
• Pathway: Cell cycle; Chronic myeloid leukemia; Colorectal cancer; Cytokine-cytokine receptor interaction; Hypertrophic cardiomyopathy (HCM); MAPK signaling pathway; Pancreatic cancer; Pathways in cancer; Renal cell carcinoma; TGF-beta signaling pathway; Hemostasis; Influenza Infection; Signaling by TGF beta
• Function: growth factor activity; protein N-terminus binding; protein heterodimerization activity; protein homodimerization activity; transcription activator activity; type II transforming growth factor beta receptor binding; type II transforming growth factor beta receptor binding