Recombinant Mouse TGFB1 Protein(Leu30-Ser390), His-tagged

• Cat.No.: TGFB1-6543M
• Product Overview: Recombinant Mouse TGFB1 Protein(Leu30-Ser390)(P04202) is expressed from HEK293 with His tag at the N-Terminus.

Specification

• Species: Mouse
• Source: HEK293
• Tag: His
• Protein Length: Leu30-Ser390
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Mouse Latent TGF beta 1, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human ITGAV&ITGB6, hFc Tag with the EC50 of 0.87μg/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 29.5/12.9 kDa. Due to glycosylation, the protein migrates to 40-50/14-15 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE
• Storage: -20 to -80°C for 12 months as supplied from date of receipt.
  -80°C for 3-6 months after reconstitution.
  2-8°C for 2-7 days after reconstitution.
  Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: Tgfb1 transforming growth factor, beta 1 [ Mus musculus ]
• Official Symbol: TGFB1
• Synonyms: TGFB1; transforming growth factor, beta 1; transforming growth factor beta-1; TGF-beta 1; TGF-beta-1; regulatory protein; transforming growth factor-beta 1; Tgfb; Tgfb-1; TGFbeta1; TGF-beta1
• Gene ID: 21803
• mRNA Refseq: NM_011577
• Protein Refseq: NP_035707