Recombinant Human TPK1 Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: TPK1-5075H
• Product Overview: TPK1 MS Standard C13 and N15-labeled recombinant protein (NP_071890) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Species: Human
• Source: HEK293
• Tag: DDK&Myc
• Description: The protein encoded by this gene functions as a homodimer and catalyzes the conversion of thiamine to thiamine pyrophosphate, a cofactor for some enzymes of the glycolytic and energy production pathways. Defects in this gene are a cause of thiamine metabolism dysfunction syndrome-5.
• Molecular Mass: 27.2 kDa
• AA Sequence: MEHAFTPLEPLLSTGNLKYCLVILNQPLDNYFRHLWNKALLRACADGGANRLYDITEGERESFLPEFINGDFDSIRPEVREYYATKGCELISTPDQDHTDFTKCLKMLQKKIEEKDLKVDVIVTLGGLAGRFDQIMASVNTLFQATHITPFPIIIIQEESLIYLLQPGKHRLHVDTGMEGDWCGLIPVGQPCSQVTTTGLKWNLTNDVLAFGTLVSTSNTYDGSGVVTVETDHPLLWTMAIKSTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: TPK1 thiamin pyrophosphokinase 1 [ Homo sapiens (human) ]
• Official Symbol: TPK1
• Synonyms: TPK1; thiamin pyrophosphokinase 1; HTPK1; placental protein 20; PP20; thiamine diphosphokinase; thiamine kinase; thiamine pyrophosphokinase 1; THMD5
• Gene ID: 27010
• mRNA Refseq: NM_022445
• Protein Refseq: NP_071890
• MIM: 606370
• UniProt ID: Q9H3S4