Recombinant Human TOLLIP, GST-tagged

• Cat.No.: TOLLIP-151H
• Product Overview: Recombinant human TOLLIP (amino acid residues 1-274), with N-terminal GST, was expressed in E.coli.

Specification

• Description: TOLLIP has an N-Terminal TOM1 binding domain (TBD) that mediates protein-protein interactions, a C2 domain that targets TOLLIP to the endosome and a C-terminal CUE domain that binds mono-ubiquitin (Lo et al., 2009). Recent studies have proposed that Interleukin 1B (IL-1B) stimulation of HEK293 cells induces aggregation of Interleukin 1 Receptors (IL-1Rs) and recruitment of MYD88 followed by the TOLLIP/IL-1 receptor-associated kinase 1 (IRAK1) complex.
• Source: E. coli
• Species: Human
• Tag: GST
• Form: 50 mM HEPES pH 7.5, 150 mM sodium chloride, 2 mM dithiothreitol, 10% glycerol
• Molecular Mass: ~57kDa
• Purity: >90% by InstantBlue™ SDS-PAGE
• Storage: 12 months at -70°C. Avoid multiple freeze/thaw cycles.
• Concentration: 0.5 mg/ml
• Protein length: 1-274 a.a.

Gene Information

• Gene Name: TOLLIP toll interacting protein [ Homo sapiens ]
• Official Symbol: TOLLIP
• Synonyms: TOLLIP; toll interacting protein; toll-interacting protein; IL 1RAcPIP; adapter protein; IL-1RAcPIP; FLJ33531
• Gene ID: 54472
• mRNA Refseq: NM_019009
• Protein Refseq: NP_061882
• MIM: 606277
• UniProt ID: Q9H0E2
• Chromosome Location: 11p
• Pathway: Cytokine Signaling in Immune system, organism-specific biosystem; IL1-mediated signaling events, organism-specific biosystem; Immune System, organism-specific biosystem; Interleukin-1 signaling, organism-specific biosystem; Signaling by Interleukins, organism-specific biosystem; Toll-like receptor signaling pathway, organism-specific biosystem; Toll-like receptor signaling pathway, organism-specific biosystem
• Function: Toll-like receptor binding; kinase binding; protein binding; signal transducer activity