Recombinant Human TNFSF8 protein, mFc-tagged

• Cat.No.: TNFSF8-5632H
• Product Overview: Recombinant Human TNFSF8 protein(P32971-1)(Gln63-Asp234), fused with N-terminal mFc tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: N-mFc
• Protein length: Gln63-Asp234
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human CD30, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Human CD30 Ligand, mFc Tag with the EC50 of 10.5ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 46.2 kDa. Due to glycosylation, the protein migrates to 62-68 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
• Storage: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
• Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: TNFSF8 tumor necrosis factor (ligand) superfamily, member 8 [ Homo sapiens ]
• Official Symbol: TNFSF8
• Synonyms: TNFSF8; tumor necrosis factor (ligand) superfamily, member 8; CD30LG; tumor necrosis factor ligand superfamily member 8; CD153; CD30-L; CD30 ligand; CD153 antigen; CD30 antigen ligand; CD30L; MGC138144
• Gene ID: 944
• mRNA Refseq: NM_001244
• Protein Refseq: NP_001235
• MIM: 603875
• UniProt ID: P32971