Recombinant Human TNFSF4 protein, His-Flag-tagged

• Cat.No.: TNFSF4-5150H
• Product Overview: Recombinant Human TNFSF4 protein(P23510-1)(Gln51-Leu183) is expressed from HEK293 with His tag and Flag tag at the N-Terminus.

Specification

• Source: HEK293
• Species: Human
• Tag: N-His-Flag
• Protein length: Gln51-Leu183
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human OX40 Ligand Trimer, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human OX40, hFc Tag with the EC50 of 37.7ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 49.7 kDa. Due to glycosylation, the protein migrates to 65-140 kDa based on Bis-Tris PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 90% as determined by Bis-Tris PAGE
• Storage: -20 to -80°C for 12 months as supplied from date of receipt.
  -80°C for 3-6 months after reconstitution.
  2-8°C for 2-7 days after reconstitution.
  Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: TNFSF4 tumor necrosis factor (ligand) superfamily, member 4 [ Homo sapiens ]
• Official Symbol: TNFSF4
• Synonyms: TNFSF4; tumor necrosis factor (ligand) superfamily, member 4; tax transcriptionally activated glycoprotein 1, 34kD , TXGP1; tumor necrosis factor ligand superfamily member 4; CD252; gp34; OX 40L; OX40L; CD134 ligand; glycoprotein Gp34; OX40 antigen ligand; TAX transcriptionally-activated glycoprotein 1; tax-transcriptionally activated glycoprotein 1 (34kD); GP34; OX4OL; TXGP1; CD134L; OX-40L
• Gene ID: 7292
• mRNA Refseq: NM_003326
• Protein Refseq: NP_003317
• MIM: 603594
• UniProt ID: P23510