Recombinant Human TNFRSF8 Protein (Met1-Lys379), His-tagged, Biotinylated

• Cat.No.: TNFRSF8-434H
• Product Overview: Recombinant Human TNFRSF8 Protein (NP_001234.2) (Met1-Lys379) was produced by HEK293 Cells expression system. This protein was expressed with a C-terminal polyhistidine tag. The purified protein was biotinylated in vitro.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: Met1-Lys379
• Description: CD3, also known as TNFRSF8, is a cell membrane protein of the tumor necrosis factor receptor (TNFR) superfamily. CD3 protein is expressed by activated, but not resting, T and B cells. CD3 can regulate proliferation of lymphocytes and may also play an important role in human immunodeficiency virus replication. As a regulator of apoptosis, CD3 protein induces cell death or proliferation, depending on the cell type, and has been shown to limit the proliferative potential of autoreactive CD8 effector T cells and protect the body against autoimmunity. CD3 protein expression is upregulated in various hematological malignancies, including Reed-Sternberg cells in Hodgkin's disease (HD), anaplastic large cell lymphoma (ALCL) and subsets of Non-Hodgkin's lymphomas (NHLs), and CD3 is also linked to leukocytes in patients with chronic inflammatory diseases, including lupus erythematosus, asthma, rheumatoid arthritis and atopic dermatitis (AD).
• Predicted N Terminal: Phe 19
• Form: Lyophilized from sterile Sterile PBS. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The recombinant TNFRSF8 consists of 372 amino acids and predicts a molecular mass of 39.9 kDa.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 90 % as determined by SDS-PAGE.
• Stability: Samples are stable for up to twelve months from date of receipt at -70 centigrade.
• Storage: Store it under sterile conditions at -20 centigrade to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4℃ before opening to recover the entire contents.
• Shipping: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
  Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Gene Information

• Gene Name: TNFRSF8 tumor necrosis factor receptor superfamily, member 8 [ Homo sapiens ]
• Official Symbol: TNFRSF8
• Synonyms: TNFRSF8; CD30, D1S166E; KI 1; Ki-1 antigen; CD30L receptor; cytokine receptor CD30; CD30; Ki-1; D1S166E
• Gene ID: 943
• mRNA Refseq: NM_001243
• Protein Refseq: NP_001234
• MIM: 153243
• UniProt ID: P28908