Recombinant Human TNFRSF11B,His-tagged

• Cat.No.: TNFRSF11B-169H
• Product Overview: Recombinant human osteoprotegerin protein, fused to His-tag at C-terminus, was expressed inHi-5 cellusing baculovirus expression system and purified by using conventional chromatography techniques. MW = 45.6 kDa (398 aa).

Specification

• Species: Human
• Source: Hi-5 Insect Cells
• Tag: His
• Description: Osteoprtegerin (OPG) is a member of the tumor necrosis factor (TNF)-related family, is referred to as TNFRSF11B and part of the OPG/receptor activator of NF-кB ligand (RANKL)/receptor activator of NF-кB (RANK) triad. This cytokine that lacks any apparent cell-association motifs and exists as a soluble secreted protein, network regulates the differentiation and activation of osteoclasts and hence the critical balance between bone formation (osteoblasts) and bone resorption (osteoclasts).
• Form: Liquid. In PBS (pH 7.4) containing 10% glycerol.
• Purity: > 85 % by SDS - PAGE.
• Concentration: 0.5 mg/ml (determined by Bradford assay).
• Sequences Of Amino Acids: ADPETFPPKY LHYDEETSHQ LLCDKCPPGT YLKQHCTAKW KTVCAPCPDH YYTDSWHTSD ECLYCSPVCK ELQYVKQECN RTHNRVCECK EGRYLEIEFC LKHRSCPPGF GVVQAGTPER NTVCKRCPDG FFSNETSSKA PCRKHTNCSV FGLLLTQKGN ATHDNICSGN SESTQKCGID VTLCEEAFFR FAVP TKFT PNW LSVLVDNLP GTKVNAESVE RIKRQHSSQE QTFQLLKLWK HQNKDQDIVK KIIQ DIDLCE NSVQRHIGHA NLTFEQLRSL MESLPGKKVG AEDIEKTIKACKPSDQILKL LSLWRIKNGD QDTLKGLMHA LKHSKTYHFP KTVTQSLKKT IRFLHSFTMY KLYQKLFLEM IGNQVQSVKI SCLSGRLVPR GSHHHHHH.
• Endotoxin Level: < 1.0 EU per1µgof protein (determined by LAL method ).
• Storage: Can be stored at +4°C short term (1 week). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles .

Gene Information

• Gene Name: TNFRSF11B tumor necrosis factor receptor superfamily, member 11b [ Homo sapiens ]
• Synonyms: TNFRSF11B, OPG, OCIF, Osteoclastogenesis inhibitory factor, TR1, MGC29565, Osteoprotegerin, TR1, Osteoclastogenesis inhibitory factor, tumor necrosis factor receptor superfamily, member 11b
• Gene ID: 4982
• mRNA Refseq: NM_002546
• Protein Refseq: NP_002537
• MIM: 602643
• UniProt ID: O00300
• Chromosome Location: 8q24
• Pathway: Cytokine-cytokine receptor interaction
• Function: cytokine activity; protein binding; receptor activity