Recombinant Human TLR5 protein, His-tagged

• Cat.No.: TLR5-2076H
• Product Overview: Recombinant Human TLR5 aa. (Leu303~Leu514 (Accession # O60602)) fused with N-terminal His tag was produced in E. coli cells.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: Leu303~Leu514
• Description: This gene encodes a member of the toll-like receptor (TLR) family, which plays a fundamental role in pathogen recognition and activation of innate immune responses. These receptors recognize distinct pathogen-associated molecular patterns that are expressed on infectious agents. The protein encoded by this gene recognizes bacterial flagellin, the principal component of bacterial flagella and a virulence factor. The activation of this receptor mobilizes the nuclear factor NF-kappaB, which in turn activates a host of inflammatory-related target genes. Mutations in this gene have been associated with both resistance and susceptibility to systemic lupus erythematosus, and susceptibility to Legionnaire disease.
• Form: Freeze-dried powder
• Molecular Mass: 30 kDa as determined by SDS-PAGE reducing conditions.
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 5.4.
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as liquid form in Phosphate buffered saline(PBS), pH 7.4.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

Gene Information

• Gene Name: TLR5 toll like receptor 5 [ Homo sapiens (human) ]
• Official Symbol: TLR5
• Synonyms: SLE1; TIL3; SLEB1; MELIOS; systemic lupus erythematosus susceptibility 1; toll/interleukin-1 receptor-like protein 3; toll-like receptor 5
• Gene ID: 7100
• mRNA Refseq: NM_003268.5
• Protein Refseq: NP_003259.2
• UniProt ID: O60602